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Determination of biogenic amines in fishery products for import and export. HPLC method
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SN/T 2209-2008
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Basic data | Standard ID | SN/T 2209-2008 (SN/T2209-2008) | | Description (Translated English) | Determination of biogenic amines in fishery products for import and export. HPLC method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X20 | | Word Count Estimation | 14,145 | | Date of Issue | 2008-11-18 | | Date of Implementation | 2009-06-01 | | Regulation (derived from) | National Quality Inspection [2008] No. 577 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard provides high-performance liquid chromatography aquatic toxic biogenic amines approach. This standard applies to shrimp, fish, shellfish and meat phenylethylamine, putrescine, cadaverine, histamine, octopamine, tyramine and spermidine detection. | SN/T 2209-2008: Determination of biogenic amines in fishery products for import and export. HPLC method
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Determination of biogenic amines in fishery products for import and export.HPLC method
Exit inspection and quarantine industry standard book People's Republic of China
Import and export of aquatic products of toxic biogenic amines
HPLC detection method
Posted 2008-11-18
2009-06-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau.
The main drafters of this standard. Fang Xiaoming, Tang Yifeng, Chen Di, Wang Chuan now, Xu Xianghua.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Import and export of aquatic products of toxic biogenic amines
HPLC detection method
1 Scope
This standard specifies the method for the determination of toxic levels of biogenic amines in aquatic products by high performance liquid chromatography.
This standard applies to shrimp, fish, shellfish meat phenylethylamine, putrescine, cadaverine, histamine, octopamine, tyramine and spermidine detection.
2 Determination
2.1 Method summary
Biogenic amines in the sample solution with perchloric acid extraction, dansyl chloride derivatization with UV detector with high performance liquid chromatography separation
Detection, external standard.
2.2 Reagents and materials
Unless otherwise specified, the reagents were of analytical grade, water is deionized water, with water as the mobile phase of ultrapure water.
2.2.1 Methanol. HPLC grade.
2.2.2 acetonitrile. HPLC grade.
2.2.3 acetone. HPLC grade.
2.2.4 Dan chloride. HPLC grade purity ≥99.0%.
2.2.5 sodium bicarbonate.
2.2.6 perchloric acid. ρ = 1.67g/mL.
2.2.7 sodium hydroxide.
2.2.8 concentrated aqueous ammonia. 25% to 28%.
2.2.9 0.4mol/L perchloric acid solution. Measure 48mL of perchloric acid (ρ = 1.67g/mL), diluted with water to 2L.
2.2.10 2mol/L sodium hydroxide solution. Weigh 4.0g of sodium hydroxide dissolved in 50mL water.
2.2.11 saturated sodium bicarbonate solution.
2.2.12 10mg/mL Dan chloride solution. Weigh 0.20g Dan chloride dissolved in 20mL acetone.
2.2.13 Standard. phenylethylamine hydrochloride (2-Phenylethylaminehydrochloride, C6H5CH2CH2NH2 · HCl, CAS.
156-28-5, purity ≥98%), and putrescine hydrochloride (Putrescinedihydrochloride, NH2 (CH2) 4NH2 · 2HCl, CAS. 333-93-
7, purity ≥98%), hydrochloric acid cadaverine (Cadaverinedihydrochloride, NH2 (CH2) 5NH2 · 2HCl, CAS. 1476-39-7, pure
Of > 98.0%), histamine dihydrochloride (Histaminedihydrochloride, C5H9N3 · 2HCl, CAS. 56-92-8, purity ≥99.0%), salt
Acid octopamine (Octopaminehydrochloride, HOC6H4CH (CH2NH2) OH · HCl, CAS. 770-05-8, purity ≥
99.0%), tyramine hydrochloride (Tyraminehydrochloride, HOC6H4CH2CH2NH2 · HCl, CAS. 60-19-5, purity ≥
99.0%), spermidine hydrochloride (Spermidinetrihydrochloride, NH2 (CH2) 3NH (CH2) 4NH2 · 3HCl, CAS. 334-50-
9, purity ≥99.5%).
2.2.14 mixed standard stock solution. Weigh accurately hydrochloride phenylethylamine, putrescine hydrochloride, hydrochloric acid, cadaverine, histamine dihydrochloride, hydrochloric acid octopamine, tyramine and hydrochloric acid
Spermidine hydrochloride standard each 0.130g, 0.183g, 0.172g, 0.166g, 0.124g, 0.127g and 0.176g in 100mL flask,
And dissolved in water to volume, to give a mixed standard stock solution 1.00mg/mL. This solution was stored at 0 ℃ ~ 4 ℃ can be used for 2 weeks.
2.2.15 working standard solution. if necessary, take the appropriate standard stock solution, diluted with water to the desired concentration.
2.3 instruments and equipment
2.3.1 HPLC with UV - visible detector or diode array detector.
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