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SN/T 2155-2008: Identification of cymbidium mosaic virus
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| SN/T 2155-2008 | English | 259 |
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Identification of cymbidium mosaic virus
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SN/T 2155-2008
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Basic data | Standard ID | SN/T 2155-2008 (SN/T2155-2008) | | Description (Translated English) | Identification of cymbidium mosaic virus | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Word Count Estimation | 10,145 | | Date of Issue | 2008-09-04 | | Date of Implementation | 2009-03-16 | | Quoted Standard | SN/T 1840 | | Regulation (derived from) | Industry standard filing Notice 2008 No. 11 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the Cymbidium mosaic virus detection and identification of the basic principles and methods. This standard applies may carry all of Cymbidium mosaic virus entry orchid seedlings, including commodity seedlings, tissue culture and virus-free seedlings and other germplasm quarantine and identification of orchids. | SN/T 2155-2008: Identification of cymbidium mosaic virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Identification of cymbidium mosaic virus
Exit inspection and quarantine industry standard book People's Republic of China
Cymbidium mosaic virus detection methods
Posted 2008-09-04
2009-03-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
The Standard Appendix B, Appendix C are normative appendices, Appendix A is an information appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. China Inspection and Quarantine Science Research Institute, People's Republic of China Shanghai Exit Inspection and Quarantine.
The main drafters of this standard. with Ning, Zhang Yongjiang, Yang Cuiyun at Chui.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Cymbidium mosaic virus detection methods
1 Scope
This standard specifies the basic principles and methods of Cymbidium mosaic virus detection identification.
This standard applies to Cymbidium mosaic virus may have all the entry orchid seedlings, including commodity seedlings, tissue culture and virus-free seedlings and other orchid
Quarantine and identification of germplasm.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
SN/T 1840 plant virus immune electron microscopy method
Principle 3
Cymbidium mosaic virus Scientific name. Cymbidiummosaicvirus, Abbreviation. CymMV. Cymbidium mosaic virus infection is a major disease of orchids
One drug, serological properties, specific coat protein is the main basis for the present standards, the taxonomic status of the virus, geographical distribution, send
Scope and main symptoms, mode of transmission, particle morphology and genomic features, etc. See Appendix A.
4 instruments, equipment and reagents
4.1 Instrument
4.1.1 transmission electron microscope.
4.1.2 enzyme-linked detector.
4.1.3 electronic balance.
4.1.4 PCR instrument.
4.1.5 gel imaging system.
4.1.6 juicer.
4.1.7 High-speed refrigerated centrifuge.
4.1.8 ultracentrifuge.
4.2 Appliances
4.2.1 adjustable pipette (0.1μL ~ 2μL, 2μL ~ 10μL, 10μL ~ 100μL, 100μL ~ 1000μL) and adjustable pipette
Head.
4.2.2 ELISA plate.
4.2.3 Eppendorf tubes.
4.2.4 mortar.
4.3 ELISA detection reagent
Sodium carbonate 1.59g
Sodium bicarbonate 2.93g
Sodium azide 0.20g
It was dissolved in 900mL of distilled water, acidified with hydrochloric pH9.6, with distilled water to make up 1L.
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