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| SN/T 2131.2-2010 | English | 239 |
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Determination of diarrheic shellfish poisons in shellfish for import and export. Part 2: Mouse biology method
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SN/T 2131.2-2010
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Basic data | Standard ID | SN/T 2131.2-2010 (SN/T2131.2-2010) | | Description (Translated English) | Determination of diarrheic shellfish poisons in shellfish for import and export. Part 2: Mouse biology method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X20 | | Classification of International Standard | 67.120.30 | | Word Count Estimation | 6,673 | | Date of Issue | 2010-01-10 | | Date of Implementation | 2010-07-16 | | Older Standard (superseded by this standard) | SN 0294-1993 | | Quoted Standard | GB/T 6682; GB 14925 | | Regulation (derived from) | National Quality Inspection [2010] No. 13 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the diarrhetic shellfish and their products in the shellfish toxin mouse bioassay. This standard applies to shellfish and their products in the diarrhetic shellfish toxin testing. | SN/T 2131.2-2010: Determination of diarrheic shellfish poisons in shellfish for import and export. Part 2: Mouse biology method
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Determination of diarrheic shellfish poisons in shellfish for import and export.Part 2. Mouse biology method
Exit inspection and quarantine industry standard book People's Republic of China
Replacing SN 0294-1993
Importers diarrhea shellfish shellfish poison test methods
Part 2. mouse bioassay
Issued on. 2010-01-10
2010-07-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 2131 "Importers Shellfish diarrhea shellfish toxin testing method" is divided into two parts.
--- Part 1. Fluorescent phosphatase inhibition assay;
--- Part 2. mouse bioassay.
This section SN/T 2131 Part 2.
This Part replaces SN 0294-1993 "export shellfish diarrhea shellfish poison test methods."
This section compared with SN 0294-1993, the main changes are as follows.
--- The original standard name for the revised "diarrheal shellfish poisons shellfish test methods - Part 2. mouse bioassay";
--- The range has been simplified and modified;
--- Increasing the terms and definitions;
--- Reagents and materials increased by 1% Tween -60 saline specific preparation methods;
--- The result of the calculation and presentation, increasing the presentation of the report;
--- Increasing the health and safety tips terms.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section was drafted by. People's Republic of China Liaoning Entry-Exit Inspection and Quarantine Yunnan People's Republic of China
Bureau, People's Republic of China Shandong CIQ.
The main drafters of this section. Juan Cao occasion, Wangqiu Yan Cheng Chiu, Xu Yang, Wang Gang, the soldiers, Zheng Huifang, Zhao Xin, Wu Zhenxing.
This Part replaces the previous editions are.
--- SN 0294-1993.
Importers diarrhea shellfish shellfish poison test methods
Part 2. mouse bioassay
1 Scope
This section SN/T 2131 specifies the method for determination of mouse bioassay shellfish and their products diarrheal shellfish toxins.
This section applies to shellfish and their products diarrheal shellfish toxin testing.
2 Normative references
The following documents contain provisions which, through reference SN/T 2131 and become part of the provisions of this section. For dated references,
All subsequent amendments (not including errata content) or revisions do not apply to this section, however, encourage the agreement on this section
Whether the parties can study the latest versions of these documents. For undated reference documents, the latest versions apply to this section.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB 14925 Laboratory Animal Facilities and Environment
3 Terms and Definitions
The following terms and definitions apply to this part of the SN/T 2131's.
3.1
Chemical structure okadaic acid (okadaicacid, referred to as OA) and its derivatives represented after ingestion can produce diarrhea-predominant
To feature present in the shellfish marine life in general toxic substances.
3.2
For weighing 16g ~ 20g of 3 ICR male mice after intraperitoneal injection of 1mL each shellfish extract, make two or three mice
24h virulence in mice died as a unit.
4 Sample preparation and preservation
4.1 Sample preservation
4.1.1 Samples should be fully representative and not individual differences as possible. At the same time, there is also sufficient number. Shelled shellfish, shelled
Or canned, etc., should take adequate number of more than 400g of meat and shellfish.
4.1.2 away from the laboratory not timely submission of samples, in addition to quality will not change at room temperature, samples should be placed in a synthetic resin
Freezer bag for inspection. As for the shell samples, the method should be 4.2 to open the shell, after the removal of the water freezing inspection.
4.2 Preparation of the sample
4.2.1 Pre-treatment samples of fresh shell
Wash thoroughly with water shellfish shell, cut the adductor muscle, open the shell, rinsed with water to remove sediment and other internal foreign matter, remove the shellfish meat.
The drug should not be heating or add a method to open the shell. Be careful not to damage other than adductor muscle tissue, especially in the midgut gland (also known as digestive diverticula, group
Woven dark green or greenish brown).
Shelled shellfish meat on the pore size of about 2mm metal sieve, drain 5min, prepared according to 4.2.3 test sample.
4.2.2 Pretreatment of frozen samples
At room temperature, the frozen samples was semi-frozen state. Shell frozen samples, cleaning method according to 4.2.1, open the shell, rinsed take the meat at this time
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