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Determination of DSP in shellfish for import and export. Part 1: Inhibition method of fluorescence phophatase activity
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SN/T 2131.1-2008
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Basic data | Standard ID | SN/T 2131.1-2008 (SN/T2131.1-2008) | | Description (Translated English) | Determination of DSP in shellfish for import and export. Part 1: Inhibition method of fluorescence phophatase activity | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X20 | | Classification of International Standard | 67.050 | | Word Count Estimation | 14,141 | | Date of Issue | 2008-09-04 | | Date of Implementation | 2009-03-16 | | Regulation (derived from) | Industry standard filing Notice No. 11 of 2008 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the quantitative detection of diarrhetic shellfish toxins in shellfish fluorescent phosphatase inhibition test methods. This standard applies to bivalve shellfish meat, shellfish column of okadaic acid and its derivatives test. | SN/T 2131.1-2008: Determination of DSP in shellfish for import and export. Part 1: Inhibition method of fluorescence phophatase activity
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Determination of DSP in shellfish for import and export.Part 1. Inhibition method of fluorescence phophatase activity
Exit inspection and quarantine industry standard book People's Republic of China
Importers and shellfish diarrhea shellfish toxin detection method
Part 1. Fluorescent phosphatase inhibition assay
Posted 2008-09-04
2009-03-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This section is SN/T 2131 Part 1.
This section of Appendix A normative appendix.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section was drafted by. People's Republic of China Liaoning Province Exit Inspection and Quarantine.
The main drafters of this section. Wu Bin, Li Ye, Wang Yuping, LI Zhen-Rong, Xie Yan.
The first part of the Department of Inspection and Quarantine issued by industry standards.
Importers and shellfish diarrhea shellfish toxin detection method
Part 1. Fluorescent phosphatase inhibition assay
1 Scope
This section SN/T 2131 provides for the quantitative detection of shellfish toxins in shellfish diarrhea fluorescence phosphatase inhibition test.
This section applies to test bivalve shellfish meat, shellfish column of okadaic acid and its derivatives.
2 Acronyms
The following abbreviations apply to this part of the SN/T 2131's.
2.1
Diarrhea shellfish toxins.
2.2
Okadaic acid.
2.3
DTX Ju
Okadaic acid derivative family.
3 Sample preparation and preservation
Save 3.1 sample
Such as laboratory tests can not immediately label should indicate the source, respectively, sampling dates, the variety and number, put a plastic bag sealed. After sampling the sample
Product should be immediately frozen at less -18 ℃.
3.2 Preparation of the sample
Wash thoroughly with water to be tested shell, open shell, thoroughly clean the interior shell, remove the internal waste quality, remove all shellfish tissue, with a filter
Paper try to dry the excess water shellfish tissue to be tested shellfish organization may not be less than 50g.
4 Determination
4.1 Method summary
Measurement principle of this method is okadaic acid (Okadaicacid, OA) and its toxic derivatives (DTXs) and their ability to inhibit serine
Acid and protein phosphatase activity of threonine directly related, in particular, PP1 and PP2A two proteins; phosphatase enzyme and a fluorescent substance in
Microplate experiments, the concentration of okadaic acid and its derivatives directly determine the ability of phosphatase enzyme hydrolysis of a fluorescent substance, after hydrolysis
Luciferase substrates fluorescence and fluorescence is read by a fluorescence microplate reader, the concentration of the toxin in the sample can be obtained by standard curve meter
Operators draw.
4.2 Reagents and materials
Unless otherwise specified, the reagents were of analytical grade, water is deionized water.
4.2.1 100% (by volume) of methanol.
4.2.2 2.5mol/L sodium hydroxide solution. 100g of sodium hydroxide dissolved in 500mL of water, dilute to 1000mL.
4.2.3 2.5mol/L hydrochloric acid solution of. 205mL concentration of 37% (volume fraction) of hydrochloric acid was added 400mL of water volume
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