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SN/T 2127-2008: Determination of β-lactam residues in foodstuffs of animal origin for import and export. MIA method
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Determination of β-lactam residues in foodstuffs of animal origin for import and export. MIA method
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SN/T 2127-2008
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Basic data | Standard ID | SN/T 2127-2008 (SN/T2127-2008) | | Description (Translated English) | Determination of ��-lactam residues in foodstuffs of animal origin for import and export. MIA method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C53;X22 | | Classification of International Standard | 67.050; 67.120.01 | | Word Count Estimation | 16,137 | | Date of Issue | 2008-09-04 | | Date of Implementation | 2009-03-16 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | Industry standard filing Notice No. 11 of 2008 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the foods of animal origin ��- lactam drug residue testing sample preparation, preservation and microbial inhibition assay method. This standard applies to milk, meat, fish and shrimp in the benzyl penicillin, ampicillin, amoxicillin, cloxacillin, dicloxacillin, oxacillin, and residues of ceftiofur and other drugs screening test, a positive result the application of other methods for confirmation. | SN/T 2127-2008: Determination of β-lactam residues in foodstuffs of animal origin for import and export. MIA method
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Determination of β-lactam residues in foodstuffs of animal origin for import and export.MIA method
Exit inspection and quarantine industry standard book People's Republic of China
Import and export of animal-derived foods β- lactams
Residue microbial inhibition assay
Posted 2008-09-04
2009-03-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard is a normative appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Fujian Exit Inspection and Quarantine, People's Republic of China Zhuhai Entry-Exit Inspection and
Quarantine Bureau.
The main drafters of this standard. Zheng Jing, Huang Xiaorong, Wu Qian, Wang Feng, Weng Guozhu, sheets of silver, Zheng Linyi, Wu Yunyun.
This standard entry-exit inspection and quarantine standards was first published.
Import and export of animal-derived foods β- lactams
Residue microbial inhibition assay
1 Scope
This standard specifies the sample preparation of foods of animal origin within β- lactam drug residue testing, preservation and inhibition of microbial detection methods.
This standard applies to milk, meat, fish and shrimp benzyl penicillin, ampicillin, amoxicillin, cloxacillin, dicloxacillin Penicillium
Su, oxacillin, penicillin and ceftiofur and other drug residue screening test, the positive results of the application of other methods for confirmation.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)
3 Sample preparation and preservation
3.1 Sample Preparation
3.1.1 Milk. Take a sample of not less than 500mL, into a clean container and marked with tags.
3.1.2 meat, fish and shrimp. Remove from the original sample part of a representative sample of the edible parts into a homogeneous high-speed masher, thoroughly mixed
Uniform, and dividing by quartering a sample of not less than 500g. Load a clean container and labeled markers.
3.1.3 sample preparation operation, should prevent sample contamination or the occurrence of the change of residue content.
3.2 Sample preservation
3.2.1 Milk. 0 ℃ ~ 4 ℃ preservation.
3.2.2 meat, fish and shrimp. -18 ℃ save the following sample, fresh or frozen tissue samples can be stored at 2 ℃ ~ 6 ℃ 72h.
4 Determination
4.1 Method summary
The sample was extracted buffer after incubation and centrifugation, the supernatant was measured. The use of antibiotics on the inhibition susceptible discriminated again
Samples contain antibiotics.
4.2 Equipment and Materials
4.2.1 thermostat. 63 ℃ ± 1 ℃.
4.2.2 centrifuge.
4.2.3 High-speed tissue masher.
4.2.4 constant temperature water bath. 80 ℃.
4.2.5 Vortex.
4.2.6 microscope. 10 × ~ 100 ×.
4.2.7 vernier caliper. Measuring range 0mm ~ 200mm, precision 0.02mm or inhibiting ring gauge.
4.2.8 sterilized dishes. diameter 90mm, bottom flat glass or plastic disposable sterile petri dish.
4.2.9 circular filter paper. diameter 10mm, thickness of 1.1mm. Japan ToyoRoshiKaisha, Ltd. Product or similar products.
4.2.10 Klinefelter bottles.
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