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Identification of Plum pox virus
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SN/T 2125-2008
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Basic data | Standard ID | SN/T 2125-2008 (SN/T2125-2008) | | Description (Translated English) | Identification of Plum pox virus | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020 | | Word Count Estimation | 8,836 | | Date of Issue | 2008-09-04 | | Date of Implementation | 2009-03-16 | | Regulation (derived from) | Industry standard filing Notice No. 11 of 2008 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the Plum pox virus quarantine and identification of the basic principles and methods. This standard applies to all with Plum pox virus entry and exit of vegetative propagation material, tissue culture, seeds and fruit quarantine and identification. | SN/T 2125-2008: Identification of Plum pox virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Identification of Plum pox virus
Exit inspection and quarantine industry standard book People's Republic of China
Plum pox virus quarantine and identification methods
Posted 2008-09-04
2009-03-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Guangdong Exit Inspection and Quarantine, Chinese Academy of Inspection and Quarantine Animal and Plant Quarantine Institute
Studies, People's Republic of China Liaoning Province Exit Inspection and Quarantine.
The main drafters of this standard. Chending Hu, Wang Zhang roots, Liming Fu Hu learn difficult MATURAL, Wang Xiufen, Feng Lixia, pipe dimensions.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Plum pox virus quarantine and identification methods
1 Scope
This standard specifies the basic principles and methods of plum pox virus quarantine and identification.
This standard applies to all vegetatively propagated material with plum pox virus entry and exit, tissue culture, quarantine and identification of seeds and fruits.
Principle 2
Plum pox virus Scientific name. Plumpoxvirus (PPV), Alias. Sharkavirus, is one of the most dangerous viruses stone fruit trees,
Particularity and specificity of nucleic acid sequences of the coat protein is the main basis of the standard; the taxonomic status of the virus, geographical distribution, send
Scope and main symptoms, transmission, serological properties and genomic characteristics, see Appendix A.
3 equipment, appliances, and reagents
3.1 Equipment
3.1.1 ELISA detector.
3.1.2 PCR amplification.
3.1.3 electrophoresis.
3.1.4 desktop high-speed refrigerated centrifuge.
3.1.5 gel imaging system.
3.1.6 electronic analytical balance.
3.2 Appliances
3.2.1 Pipette (0.1μL ~ 2μL, 2μL ~ 10μL, 10μL ~ 100μL, 100μL ~ 1000μL).
3.2.2 mortar.
3.2.3 0.2mL, 0.5mL, 1.5mL tubes.
3.2.4 ELISA plate.
3.3 ELISA detection reagent
Sodium carbonate (Na2CO3) 1.59g
Sodium bicarbonate (NaHCO3) 2.93g
Sodium azide (NaN3) 0.2g
Was dissolved in 1000mL of distilled water, the solution pH was 9.6, it does not need to adjust the pH.
Sodium chloride (NaCl) 8g
Potassium dihydrogen phosphate (KH2PO4) 0.2g
Disodium hydrogen phosphate (Na2HPO4 · 12H2O) 2.9g
Potassium chloride (KCl) 0.2g
Sodium azide (NaN3) 0.2g
Dissolved in sterile water and dilute to 1000mL.
Diethyl carbamide (DIEC A) 2.0g
PVP-10 20.0g
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