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SN/T 2124-2013: Quarantine protocol for infection with Gyrodactylus safaris
Status: Valid SN/T 2124: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2124-2013 | English | 419 |
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Quarantine protocol for infection with Gyrodactylus safaris
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SN/T 2124-2013
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| SN/T 2124-2008 | English | 559 |
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The Atlantic salmon Gyrodactylus quarantine technical specifications
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SN/T 2124-2008
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PDF similar to SN/T 2124-2013
Basic data | Standard ID | SN/T 2124-2013 (SN/T2124-2013) | | Description (Translated English) | Quarantine protocol for infection with Gyrodactylus safaris | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 07.080 | | Word Count Estimation | 16,117 | | Older Standard (superseded by this standard) | SN/T 2124-2008 | | Quoted Standard | GB/T 6682; GB/T 18088 | | Regulation (derived from) | AQSIQ notification issued in 2013 on the first batch of 179 entry-exit inspection and quarantine of industry standards; industry standard for filing Notice 2013 No. 9 (No. 165 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the Atlantic salmon when three generations of coccidiosis quarantine sample collection, morphological examination, polymerase chain reaction (PCR) screening and identification of species. This standard applies to the three generati | SN/T 2124-2013: Quarantine protocol for infection with Gyrodactylus safaris---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for infection with Gyrodactylus safaris
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Instead of the SN/T 2124-2008
Atlantic salmon three generations of echinococcosis quarantine Technical Specifications
Issued on. 2013-03-01
2013-09-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
The standard reference to the World Organisation for Animal Health (OIE) of "aquatic animal disease diagnostic manual (2011 Edition)" on 2.3.03 Atlantic salmon
Relevant sections of three generations of echinococcosis, combined with data reported in the literature as well as the drafting of many years of work experience in quarantine at home and abroad.
Instead of the standard SN/T 2124-2008 "Atlantic salmon Gyrodactylus quarantine technical specifications."
This standard compared with SN/T 2124-2008, the main technical changes are as follows.
--- Simplifies the morphological identification methods, no longer used as the main basis for the determination of the species;
--- Remove the IGS sequences by PCR method;
--- Increase Atlantic salmon Gyrodactylus chart, mark the subsequent absorption structure, increase digestion and parasite species identification based hook sickle
method.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Chinese Academy of Inspection and Quarantine, Shenzhen People's Republic of China Exit Inspection and Quarantine, the People's Republic of China
State and Shandong Entry-Exit Inspection and Quarantine, Ningbo People's Republic of China.
The main drafters of this standard. Wushao Jiang, Lin Xiangmei Liu Orientin, Caixia, Lvji Zhou, Yue Zhiqin, Liu, Wang Jianfeng, Zhang Yongning, Jia Guangle,
Zhang Min, Deng Jun flower.
This standard replaces the standards previously issued as follows.
--- SN/T 2124-2008.
Atlantic salmon three generations of echinococcosis quarantine Technical Specifications
1 Scope
This standard specifies the Atlantic salmon three generations of echinococcosis quarantine when sample collection, morphological examination, polymerase chain reaction (PCR) screening and species
Identification of class.
This standard applies to the three generations of Atlantic salmon echinococcosis quarantine and epidemiological investigation.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 18088 Entry and Exit Animal Quarantine sampling
3 Abbreviations
The following abbreviations apply to this document.
ITS. amplified internal transcribed spacer (internaltranscribedspacer)
COⅠ. mitochondrial cytochrome oxidase Ⅰ gene (mitochondrialcytochromeCoxidaseⅠ)
4 pathogen Overview
Atlantic salmon Gyrodactylus (Gyrodactylussalaris) were responsible for a three generations of Atlantic salmon viviparous freshwater parasite coccidiosis. From the morphological
Point of view, and belong to the Atlantic salmon Gyrodactylus Gyrodactylus G.teuchis (mainly parasitic on brown trout, Atlantic salmon and rainbow trout) and G.thymalli (main
To parasite in trout) are very similar, easily confused with each other, the specific circumstances see Appendix A.
5 material
Unless otherwise specified, the use of chemical reagents were of analytical grade. Test water required to achieve the GB/T 6682 in a water requirements.
5.1 Ammonium bitterness - glycerol (APG) solution. preparation methods, see Appendix B, B.1.
5.2 soft tissue digestion, digestive stop solution. preparation methods see B.2, B.3.
5.3 lysate. preparation methods, see B.4.
5.4 Primer. concentration 10μmol/L, which sequence is as follows.
ITS-up. 5'-TTTCCGTAGGTGAACCT-3 '
ITS-lp. 5'-TCCTCCGCTTAGTGATA-3 '
COⅠ-up. 5'-TAATCGGCGGGTTCGGTAA-3 '
COⅠ-lp. 5'-GAACCATGTATCGTGTAGCA-3 '
5.5 PCR buffer, dNTP, DNA polymerase, DNAMarker, electrophoresis sample buffer and the like, are commercially kit components.
5.6 electrophoresis buffer. preparation methods see B.5.
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