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SN/T 2097-2008: Codes for examination of monkeypox at entry-exit port
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2097-2008 | English | 169 |
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Codes for examination of monkeypox at entry-exit port
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SN/T 2097-2008
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Basic data | Standard ID | SN/T 2097-2008 (SN/T2097-2008) | | Description (Translated English) | Codes for examination of monkeypox at entry-exit port | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C62 | | Word Count Estimation | 6,619 | | Date of Issue | 2008-07-17 | | Date of Implementation | 2009-02-01 | | Regulation (derived from) | Industry standard filing Notice 2008 No. 9 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the entry and exit points of entry and exit of personnel monkeypox virus laboratory test methods and results reporting, including sample collection, processing, testing procedures, methods, results and reports is determined. This standard applies to the entry and exit points of monkeypox virus laboratory. | SN/T 2097-2008: Codes for examination of monkeypox at entry-exit port---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Codes for examination of monkeypox at entry-exit port
Exit inspection and quarantine industry standard book People's Republic of China
Entry-exit inspection of personnel monkeypox
Posted 2008-07-17
2009-02-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Guangdong Entry-Exit Inspection and Quarantine Huizhou People's Republic of China
Bureau, China CDC viral disease Control and Prevention.
The main drafters of this standard. Xing Lu Qin, Li Xiaobo, Zhong Yuqing, Nguyen force, Xia Yong division, Gobo rotation, Huang Ji City, Hong Ye, phase Dapeng Zheng Kui.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Entry-exit inspection of personnel monkeypox
1 Scope
This standard specifies the entry and exit ports of entry and exit of personnel monkeypox virus laboratory test methods and results reports, including sample collection, at the
Processing, testing procedures, methods, and determines and reports.
This standard applies to the entry and exit ports monkeypox virus laboratory.
2 Terms and definitions
The following terms and definitions apply to this standard.
2.1
Man and beast by the monkeypox virus (monkeypoxvirus) were suffering caused by infectious diseases, the main clinical manifestations of high fever, headache, back pain, the body does not
Fitness, cough, swollen lymph nodes, associated with systemic rash appears similar to smallpox, occasional abdominal pain.
2.2
Real-time PCR method is based on the conventional PCR, to add a specific fluorescent probe. The oligonucleotide probes period
Acid ends were marked with a reporter group and a quencher group. When the probe is intact, the fluorescence signal emitted by the reporter group quencher
Mission absorption; PCR amplification using T Rao q enzyme 5'-3 'exonuclease activity of the enzyme degradation of the probe, so that the report fluorophore and quencher points
Off, thereby fluorescence monitoring system can receive the fluorescent signal, that is, each amplified a DNA strand, there is a fluorescent molecule is formed to achieve a fluorescent
PCR product accumulation and formation of the optical signal completely synchronized.
2.3
The number of cycles when fluorescence signal of each reaction tube reaches a set threshold experienced.
3 instruments and equipment
We need the following equipment.
--- Roche quantitative PCR instrument;
---Biological safety cabinets;
--- Autoclave;
--- Ordinary refrigerator;
--- Ordinary desktop centrifuge (centrifugal cup with security seal);
--- Refrigerated centrifuge (centrifugal cup with security seal);
--- Mortar;
--- Vortexer.
4 Reagents
4.1 nucleic acid extraction reagent. Use TakaRa genomic DNA extraction kit 1), see instructions.
4.2 Hot-StartMixmaster reaction kit was purchased from Roche, 1).
1) provided by the designated units, this information is given for the convenience of users of this standard does not imply endorsement of the product. If other equivalent product with
Have the same effect, you can use these equivalent products.
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