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SN/T 2080-2008: Oak tree sudden death germs quarantine and identification methods
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2080-2008 | English | 319 |
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Oak tree sudden death germs quarantine and identification methods
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SN/T 2080-2008
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Basic data | Standard ID | SN/T 2080-2008 (SN/T2080-2008) | | Description (Translated English) | Oak tree sudden death germs quarantine and identification methods | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Word Count Estimation | 12,166 | | Date of Issue | 2008-04-29 | | Date of Implementation | 2008-11-01 | | Regulation (derived from) | Industry standard filing Notice 2008 No. 7 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the oak tree in the sudden death phytosanitary quarantine and identification of bacteria. This standard applies to the host bacteria for sudden oak tree seedlings, logs, bark, branches, leaves and other plant parts and soil and media, as well as lumber, wood packaging and wood products man entry quarantine. | SN/T 2080-2008: Oak tree sudden death germs quarantine and identification methods---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Oak tree sudden death germs quarantine and identification methods
Exit inspection and quarantine industry standard book People's Republic of China
The disease is fond quarantine and identification methods
Posted 2008-04-29
2008-11-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
The Standard Appendix A, Appendix B, Appendix C and Appendix D is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Chinese Academy of Inspection and Quarantine.
Drafters of this standard. Wu Shan goods, Yan Jin, Liaotai Lin, Wu Yan.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
The disease is fond quarantine and identification methods
1 Scope
This standard specifies the sudden death of oak trees in the phytosanitary quarantine and identification of bacteria.
This standard applies for the disease is fond of host nursery stock, logs, bark, branches, leaves and other plant parts and soil and the media,
And wood, wood packaging and wood products entry quarantine.
Principle 2
2.1 taxonomic status
English name. pathogenofsuddenoakdeath, pathogenoframorumleafblight
He said SOD), as well as azaleas, flowers, pods and other dieback (TwigBlightofRhododendron).
Germs in the world and the distribution of host plants, see Appendix A and Appendix B.
2.2 Identification of principle
According disease is fond Damage symptoms on the host, morphological properties, nested PCR amplified fragment as a specific identification basis.
3 apparatus and appliances
3.1 Instrument
3.1.1 biological microscope, stereo microscope.
3.1.2 Light biological incubator.
3.1.3 autoclave.
3.1.4 High-speed centrifuge.
3.1.5 PCR amplification, electrophoresis, gel imager.
3.2 Appliances
3.2.1 plastic box.
3.2.2 black light (18W, 320nm ~ 380nm).
3.2.3 plastic pestle pipette.
4 Reagents and culture medium
4.1 Reagents
4.1.1 agar, V8 liquid, carrots, rhododendron leaves.
4.1.2 50% of the horses neomycin, ampicillin sodium, sodium rifampicin, PCNB, calcium carbonate.
4.1.3 SDS, Tris-HCl, magnesium chloride, hydrochloric acid, EDTA, sodium hydroxide, sodium acetate, potassium chloride, Tris, CTAB, TBE, ethanol,
Phenol, ethidium bromide (EB), chloroform, isopropanol, isoamyl alcohol.
4.1.4 proteinase K, T Rao q polymerase, dNTP, DNA molecular weight Marker (100bp).
4.1.5 Nested PCR primers Phyto1/Phyto4, Phyto2/Phyto3.
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