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Determination of tylosin residues in royal jelly for import and export. Enzyme-linked immunosorbent assay
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SN/T 2060-2008
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Basic data | Standard ID | SN/T 2060-2008 (SN/T2060-2008) | | Description (Translated English) | Determination of tylosin residues in royal jelly for import and export. Enzyme-linked immunosorbent assay | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Word Count Estimation | 15,123 | | Date of Issue | 2008-04-29 | | Date of Implementation | 2008-11-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | Industry standard filing Notice 2008 No. 7 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the royal jelly and royal jelly lyophilized powder tylosin residues enzyme immunoassay method. This standard applies to royal jelly and royal jelly lyophilized powder tylosin residues. | SN/T 2060-2008: Determination of tylosin residues in royal jelly for import and export. Enzyme-linked immunosorbent assay
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Determination of tylosin residues in royal jelly for import and export.Enzyme-linked immunosorbent assay
Exit inspection and quarantine industry standard book People's Republic of China
Importers and tylosin residues in royal jelly
Determination ELISA
Posted 2008-04-29
2008-11-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Zhejiang Exit Inspection and Quarantine.
The main drafters of this standard. Shi Weiliang, Zhang Xiaofeng, Lihao Yan, Zhu Zhenjiang, He Yongqiang.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Importers and tylosin residues in royal jelly
Determination ELISA
1 Scope
This standard specifies the method of enzyme-linked immunoassay and royal jelly royal jelly lyophilized tylosin residues.
This standard applies to the determination of tylosin residue in royal jelly and lyophilized royal jelly.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequently
Some amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Study whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987)
3 Method summary
And adjusted to this standard acidic heptane sulfonate buffer to extract tylosin residue in royal jelly, royal jelly protein precipitation
pH 7.0. After processing the samples remaining enzyme-labeled tylosin and tylosin jointly compete for binding to the coated sheep on a microplate
Anti-rabbit antibody, to remove unbound enzyme-labeled tylosin and tylosin by washing, followed by addition of chromogenic substrate, the absorbance was measured with a microplate reader
Degrees, according to the content of the sample absorbance worth tylosin.
4 Reagents and materials
Unless otherwise indicated, all reagents were of analytical grade water as a water GB/T 6682 regulations.
4.1 tylosin detection kit (see Appendix A).
4.2 heptane sulfonate buffer solution. Weigh 10.1g heptane sulfonate [CH3 (CH2) 6SO3Na], 11.4g sodium phosphate (Na3PO4 ·
12H2O) dissolved in water and made up to 1L, with phosphoric acid to adjust the pH to 2.0.
4.3 phosphate buffer solution. Weigh sodium phosphate (Na2HPO3 · 12H2O) 34g, dissolved in water and made up to 1L, pH was adjusted to 8.0.
4.4 HLB cartridge, Oasis (or equivalent), 3mL (60mg).
4.5 tylosin Standard. 98% or greater purity.
4.6 tylosin preparation of standard solution. methanol as the solvent formulated to 400μg/mL of the stock solution at -20 ℃ conditions
save.
5. Apparatus
5.1 microplate reader.
5.2 eight pipettes. 10μL ~ 100μL.
5.3 Single channel pipettes. 10μL ~ 100μL, 100μL ~ 1000μL and 2mL ~ 10mL.
5.4 hybrid oscillator.
5.5 low-speed centrifuge. 6000r/min.
5.6 stoppered test tube. 50mL.
5.7 Electronic Balance. 0.01g ~ 100g.
5.8 pH meter.
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