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Determination of chloramphenicol residues in royal jelly for import and export. Enzyme-linked immunosorbent assay
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SN/T 2058-2008
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Basic data | Standard ID | SN/T 2058-2008 (SN/T2058-2008) | | Description (Translated English) | Determination of chloramphenicol residues in royal jelly for import and export. Enzyme-linked immunosorbent assay | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Word Count Estimation | 16,197 | | Date of Issue | 2008-04-29 | | Date of Implementation | 2008-11-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | Industry standard filing Notice 2008 No. 7 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the lyophilized royal jelly and chloramphenicol residues in enzyme-linked immunoassay. This standard applies to royal jelly and lyophilized chloramphenicol residues. | SN/T 2058-2008: Determination of chloramphenicol residues in royal jelly for import and export. Enzyme-linked immunosorbent assay
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Determination of chloramphenicol residues in royal jelly for import and export.Enzyme-linked immunosorbent assay
Exit inspection and quarantine industry standard book People's Republic of China
Importers and royal jelly Determination of chloramphenicol residues
ELISA
Posted 2008-04-29
2008-11-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Zhejiang Exit Inspection and Quarantine.
The main drafters. Zhang Xiaofeng, Shi Weiliang, Zhu Zhenjiang, Fang Ying, Dong Qiang.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Importers and royal jelly Determination of chloramphenicol residues
ELISA
1 Scope
This standard specifies the method of enzyme-linked immunoassay and royal jelly freeze-dried powder of chloramphenicol residues.
This standard applies to the determination of chloramphenicol residues in royal jelly and lyophilized powder.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987)
3 Method summary
This standard TCA to precipitate the protein and royal jelly extract residues of chloramphenicol, and then HLB purification. Microplates
Coated with sheep anti-rabbit IgG antibody was added specific antibody (rabbit anti-chloramphenicol antibody), an enzyme-labeled chloramphenicol, chloramphenicol reference sample or standard
After taking liquid, with specific antibody coated sheep anti-rabbit IgG antibody, while the free enzyme-labeled chloramphenicol and chloramphenicol competitive and special
Specific antibody binding. Remove unbound enzyme-labeled chloramphenicol and chloramphenicol by washing, followed by addition of chromogenic substrate, the absorbance was measured with a microplate reader
Degrees, according to the absorbance of the sample is worth chloramphenicol content.
4 Reagents and materials
Unless otherwise indicated, all reagents were of analytical grade water as a water GB/T 6682 regulations.
4.1 chloramphenicol detection kit (see Appendix A).
4.2 methanol.
4.3 5% TCA. 5.0g TCA with dissolved in water and dilute to 100mL.
4.4 30% methanol. 30.0mL of methanol (4.2) with water volume to 100mL.
4.5 isooctane - a mixture of chloroform. iso-octane, chloroform mixed to 2.3 (volume ratio).
4.6 HLB cartridge. Oasis (or equivalent), 3mL (60mg).
Chloramphenicol 4.7 standard. Purity ≥98%.
Preparation of standard solution of chloramphenicol 4.8. Weigh 0.01g chloramphenicol standard to methanol to 100mL, the preparation of 100μg/mL of
The working fluid at 4 ℃ ~ 8 ℃ preservation conditions.
4.9 blank sample. selection is not exactly known royal jelly samples containing chloramphenicol, as a blank sample. With samples for testing spiked prison
Control experiments.
5 Instruments
5.1 microplate reader.
5.2 8 pipettes. 10μL ~ 100μL.
5.3 Single channel pipettes. 10μL ~ 100μL, 20μL ~ 200μL, 100μL ~ 1000μL and 2mL ~ 10mL.
5.4 hybrid oscillator.
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