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SN/T 2039-2007: Identification of Mediterranean fruit fly, ceratitis capitata (Wiedemann). PCR protocol
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Identification of Mediterranean fruit fly, ceratitis capitata (Wiedemann). PCR protocol
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SN/T 2039-2007
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Basic data | Standard ID | SN/T 2039-2007 (SN/T2039-2007) | | Description (Translated English) | Identification of Mediterranean fruit fly, ceratitis capitata (Wiedemann). PCR protocol | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.080 | | Word Count Estimation | 8,855 | | Date of Issue | 2007-12-24 | | Date of Implementation | 2008-07-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | Industry standard filing Notice 2008 No. 2 (No. 98 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the Mediterranean fruit fly conventional PCR and SYBR Green real-time PCR for identification. This standard applies to the entry of fruit, nightshade vegetables (including tomatoes, eggplant and peppers, etc.) and packaging materials, soil, etc., Inspection and Quarantine of the Mediterranean fruit fly eggs, larvae, pupae and adults of the species identification. | SN/T 2039-2007: Identification of Mediterranean fruit fly, ceratitis capitata (Wiedemann). PCR protocol
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Identification of Mediterranean fruit fly, ceratitis capitata (Wiedemann) .PCR protocol
Exit inspection and quarantine industry standard book People's Republic of China
Mediterranean fruit fly quarantine and identification of
PCR Method
Posted 2007-12-24
2008-07-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China SZCIQ, People's Republic of China Guangdong Entry-Exit Inspection and
Quarantine Bureau.
The main drafters of this standard. Yang Weidong, Yudao Jian, Hu learning difficult, Conlin, Chen Chi-nan, Zhong Jianzhong, Zhang Guiming, Xu Lang.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Mediterranean fruit fly quarantine and identification of
PCR Method
1 Scope
This standard specifies the Mediterranean fruit fly SYBRGreen conventional PCR and real-time PCR identification method.
This standard applies to the entry of fruit, Solanaceae vegetables (including tomatoes, eggplants and peppers, etc.) and packaging, such as inspection and quarantine soil ground
Sea fruit fly eggs, larvae, pupae and adults of the species identification.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequently
Some amendments (excluding corrections) or revisions do not apply to this standard, however, encourage the parties to reach an agreement based on the study criteria
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 6682 analytical laboratory use specifications and test methods (neq ISO 3696)
3 Abbreviations
The following abbreviations apply to this standard.
3.1
SYBRGreen real-time fluorescence PCR.
3.2
Deoxynucleoside triphosphates.
3.3
Melting curve analysis.
3.4
T Rao enzyme q
Thermus aquaticus DNA polymerase.
3.5
Cytochrome oxidase subunit I.
3.6
Cycle threshold.
Principle 4
Fly samples (egg, larva, pupa or adult) after extraction of total genomic DNA, the use of specific primers designed by conventional PCR
SYBRGreen and real-time fluorescence PCR (SG-PCR) technique, specific amplification Mediterranean fruit fly mtDNA COI gene fragment
(343bp), according to the results of PCR amplification (conventional PCR product by gel electrophoresis; SG-PCR by amplification curve and melting curve),
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