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SN/T 2022-2007: Protocol of polymerase chain reaction (PCR) for Eperythrozoon wenyoni
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2022-2007 | English | 259 |
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Protocol of polymerase chain reaction (PCR) for Eperythrozoon wenyoni
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SN/T 2022-2007
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Basic data | Standard ID | SN/T 2022-2007 (SN/T2022-2007) | | Description (Translated English) | Protocol of polymerase chain reaction (PCR) for Eperythrozoon wenyoni | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 8,880 | | Date of Issue | 2007-12-24 | | Date of Implementation | 2008-07-01 | | Regulation (derived from) | Industry standard filing Notice 2008 No. 2 (No. 98 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the temperature Eperythrozoon polymerase chain reaction method. This standard applies to Winchester Eperythrozoon identification and detection. | SN/T 2022-2007: Protocol of polymerase chain reaction (PCR) for Eperythrozoon wenyoni---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of polymerase chain reaction (PCR) for Eperythrozoon wenyoni
Exit inspection and quarantine industry standard book People's Republic of China
Niu Wen Eperythrozoon polymerase chain reaction
Practice
Posted 2007-12-24
2008-07-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard is a normative appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau, Huazhong Agricultural University.
The main drafters of this standard. Li Shuqing, Hu Yongqiang, Zhao Junlong, Wang Qiao whole, Zhifei Chen, Duque, WangGuiJiang, Huan-Zhang Liu.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Niu Wen Eperythrozoon polymerase chain reaction
Practice
1 Scope
This standard specifies the Winchester Eperythrozoon polymerase chain reaction method.
This standard applies to the identification and detection Eperythrozoon body.
2 Overview
mwF/mwR3, can be amplified by PCR fragment of 738bp for identification and detection Eperythrozoon body.
3 equipment and materials
3.1 Equipment
3.1.1 PCR amplification.
3.1.2 electrophoresis.
3.1.3 gel imaging system.
3.1.4 microwave.
3.1.5 adjustable pipette (2μL, 10μL, 100μL, 200μL, 1000μL).
3.1.6 centrifuge.
3.1.7 scales (0.0001g).
3.1.8 water bath.
3.2 Supplies
3.2.1 PCR reaction tube.
3.2.2 1.5mL tubes.
3.2.3 pipette dropper.
3.3 Reagents
3.3.1 triple-distilled water 0.103MPa sterilization 15min.
3.3.2 agarose.
3.3.3 ethidium bromide.
3.3.4 T Rao q enzyme (5U/μL).
3.3.5 10 × PCR reaction buffer.
3.3.6 magnesium chloride (MgCl2) 25mmol/L.
3.3.7 1mmol/LdNTP.
3.3.8 Phenol - chloroform - isoamyl alcohol (25.24.1).
3.3.9 Molecular Weight indicator. 100bp ~ 2000bpDNAMarker.
3.3.10 50mg/mL lysozyme, 10% SDS, 10mg/mL proteinase K, 5mol/L sodium chloride (NaCl), 5% cetyltrimethylammonium
Bromide (CTAB), 2mol/L sodium acetate (NaAc), 70% ethanol, 10mg/mL ethidium bromide (EB), Tris- acetate electrophoresis buffer
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