|
US$679.00 · In stock
Delivery: <= 4 days. True-PDF full-copy in English will be manually translated and delivered via email.
SN/T 2021-2007: Protocol of quarantine for bovine anaplasmosis
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2021-2007 | English | 679 |
Add to Cart
|
4 days [Need to translate]
|
Protocol of quarantine for bovine anaplasmosis
| Valid |
SN/T 2021-2007
|
PDF similar to SN/T 2021-2007
Basic data | Standard ID | SN/T 2021-2007 (SN/T2021-2007) | | Description (Translated English) | Protocol of quarantine for bovine anaplasmosis | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 21,268 | | Date of Issue | 2007-12-24 | | Date of Implementation | 2008-07-01 | | Quoted Standard | GB/T 18651; SN/T 1679 | | Adopted Standard | OIE(2204), NEQ | | Regulation (derived from) | ?National-accreditation-Science [2011] 63 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the bovine anaplasmosis quarantine technical specifications, including smear microscopy, polymerase chain reaction, enzyme-linked immunosorbent assay method. This standard applies to bovine anaplasmosis quarantine. | SN/T 2021-2007: Protocol of quarantine for bovine anaplasmosis---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of quarantine for bovine anaplasmosis
Exit inspection and quarantine industry standard book People's Republic of China
Bovine anaplasmosis quarantine Technical Specifications
Posted 2007-12-24
2008-07-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Table of Contents
Introduction Ⅲ
1 Scope 1
2 Normative references 1
3 1 Overview
4 Diagnosis 1
4.1 1 clinical symptoms and pathological changes
4.2 Pathogen Identification 2
4.3 serology 5
Appendix A (informative) bovine anaplasmosis Overview 9
Appendix B (normative) slides processing and Giemsa staining solution preparation 11
Formulated Appendix C (Normative Appendix) PCR reagent 12
Preparation of Annex D (normative) serological reagents 15
Foreword
This standard is a reference to the International Organization for Animal Health (OIE) "ManualofDiagnostic, TestsandVaccinesforTerrestrial
Animals "(2004 edition) CHAPTER2.3.7BOVINEANAPLASMOSIS, at home and abroad a large number of references and standards
Bovine anaplasmosis research diagnostic method of drafting units prepared.
The Standard Appendix B, Appendix C, Appendix D is a normative appendix, Appendix A is an information appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Central China
Agricultural University.
The main drafters of this standard. Li Shuqing, Zhifei Chen, Ho Kwok Sing, Yao Baoan, the Xuan Wu Jian three WangGuiJiang, Duque.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Bovine anaplasmosis quarantine Technical Specifications
1 Scope
This standard specifies the bovine anaplasmosis quarantine technical specifications, including smear microscopy, polymerase chain reaction, enzyme-linked immunosorbent assay detection
Measuring method.
This standard applies to bovine anaplasmosis quarantine.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 18651 bovine anaplasmosis rapid agglutination detection method
SN/T 1679 animal anaplasmosis micro complement fixation test procedures
3 Overview
Bovine anaplasmosis (Bovineanaplasmosis) by Anaplasma parasites in cattle red blood cells, causing fever, anemia, jaundice and progressive
Weight loss, or even a disease death. The distribution of the disease, pathogen classification, the media, etc. See Appendix A.
Diagnosis of bovine anaplasmosis include clinical symptoms and pathological changes, pathogen identification and serum antibodies. Serological detection methods
The complement fixation test (Complementfixationtest, CFT) and card agglutination test (Cardagglutinationtest, CAT) is
The most commonly used method. CFT used for many years, the rules see SN/T 1679, but recent data confirm the diagnosis of persistent infection in CFT
When the cow, the sensitivity is only 20%, most of the carriers of the pathogen can not be detected. Therefore, CFT confirmatory test for individual animals is not reliable
of. The advantages of CAT is sensitive, rapid, both in the laboratory can also be carried out in the field, the test results can be reached in a few minutes. False negative rate
It is very low, but non-specific reaction is a problem. It can be used as screening methods, operating procedures, see GB/T 18651. Competitive ELISA method in the detection sensitivity
Infected animals, which greatly increase the sensitivity. Indirect ELISA, dot ELISA and fluorescent antibody test can also be used to detect antibodies specific, but
Since an indirect fluorescent antibody test to detect the operation of their day limited number of samples, and there are non-specific fluorescence reaction, generally wish to vote
Other serological methods.
4 Diagnosis
4.1 clinical symptoms and pathological changes
Anaplasmosis with increasing age of the animal becomes severe, infected cattle showed temperature continues to rise, reaching 10d ~ 21d, but rarely exceed
Over 41 ℃, and accompanied by anemia, weakness and breathing difficulties, especially increased after exercise. Early depression, loss of appetite is not obvious, but with
Development of the course and became clear. The most typical clinical symptoms of anemia and jaundice, which occurs late in the disease. Hemoglobin does not appear
White anemia and hemoglobinuria, but because of bile pigments in the urine, infected cattle appear brown urine, that contribute to the differential diagnosis of anaplasmosis and
Babesiosis. This disease is often in the same area two popular, can only be diagnosed through pathogen identification. In addition, when the number of Anaplasma in blood smears
A relatively small amount (such as in the recovery stage of the disease), red blood cell count and hematocrit decrease diagnosis Anaplasma infection. No edge
Slurry virulent, when an outbreak of cattle mortality rate of up to 50% to 80%. Miscarriage pregnant cows, breeding cows milk production reduced by 30%
~ 50%, or even stop lactation. Beef cattle weight has dropped a lot, so that the quality and quantity of livestock suffered serious losses. Autopsy performance
The anemia and weight loss, jaundice, liver, spleen, organs, jaundice, biliary thick, sometimes bleeding heart, dark urine than normal. The disease is hepatic necrosis
Characteristics. Central Anaplasma pathogenic weak, less affected bovine infection.
|