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| SN/T 1996-2007 | English | 479 |
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Detemination of diarrhetic shellfish poison in shellfish. ELISA method
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SN/T 1996-2007
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PDF similar to SN/T 1996-2007
Basic data | Standard ID | SN/T 1996-2007 (SN/T1996-2007) | | Description (Translated English) | Detemination of diarrhetic shellfish poison in shellfish. ELISA method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X20 | | Classification of International Standard | 07.100.30 | | Word Count Estimation | 12,131 | | Date of Issue | 2007-12-24 | | Date of Implementation | 2008-07-01 | | Quoted Standard | SN 0294 | | Regulation (derived from) | Industry standard filing Notice 2008 No. 2 (No. 98 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the diarrhetic shellfish toxins in shellfish sampling, sample preparation and enzyme-linked immunosorbent assay. This standard applies to edible parts of bivalve seafood diarrheal shellfish toxin testing. | SN/T 1996-2007: Detemination of diarrhetic shellfish poison in shellfish. ELISA method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detemination of diarrhetic shellfish poison in shellfish.ELISA method
Exit inspection and quarantine industry standard book People's Republic of China
Diarrhea shellfish shellfish poison test methods
Enzyme-linked immunosorbent assay
Posted 2007-12-24
2008-07-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Liaoning Province Exit Inspection and Quarantine.
The main drafters of this standard. the soldiers, Cao Juan occasion, Gao light, linen Lidan, Jindong right, Xie Yan, Li Li, Sun Jie.
This is the first issue of inspection and quarantine industry standards.
Diarrhea shellfish shellfish poison test methods
Enzyme-linked immunosorbent assay
1 Scope
This standard specifies the sampling, sample preparation and method of enzyme-linked immunoassay diarrhea shellfish toxin testing.
This standard applies to edible portions of bivalve seafood diarrhea Shellfish Toxin test.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
SN 0294 export shellfish shellfish toxin testing method diarrhea
3 definitions, terms and abbreviations
3.1 Definitions and Terminology
The following terms and definitions apply to this standard.
3.1.1
Chemical structure okadaic acid (okadaic, acid, referred to as OA) and its derivatives represented after ingestion can produce diarrhea as
The main features present in the shellfish marine life in general toxic substances.
3.2 Acronyms
3.2.1
Diarrhea shellfish toxins.
3.2.2
Enzyme-linked immunosorbent assay.
4 Sampling and sample preparation
Referring SN 0294 regulations.
5 measurement method
5.1 Principle of Measurement
Determination of the basis of this method is a competitive enzyme immunoassay, free of diarrhea and diarrheal shellfish poisons shellfish toxin enzyme markers competition
Fight diarrhea shellfish toxin antibody is not bound enzyme label is removed in the washing step. The enzyme substrate and chromogenic agent is added to the wells
And incubation, the bound enzyme markers will be colorless chromogenic agent into a blue product was added to the reaction solution after the termination of the color change from blue to yellow
color. Measuring the absorbance of the solution in the pores of 450nm wavelength microplate reader, the sample solution and diarrheal shellfish poisons into absorbance
Inversely, drawn by the quantitative calibration curve.
5.2 Reagents and materials
Unless otherwise specified, all chemical reagents were of analytical grade, water re-distilled water.
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