| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1966-2007 | English | 479 |
Add to Cart
|
4 days [Need to translate]
|
Determination of chloramphenicol residues in aquatic product. Radio-receptor assay method
| Obsolete |
SN/T 1966-2007
|
PDF similar to SN/T 1966-2007
Basic data | Standard ID | SN/T 1966-2007 (SN/T1966-2007) | | Description (Translated English) | Determination of chloramphenicol residues in aquatic product. Radio-receptor assay method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X20 | | Classification of International Standard | 67.050 | | Word Count Estimation | 12,171 | | Date of Issue | 2007-08-06 | | Date of Implementation | 2008-03-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | Accredidation-Technology-Letter [2015] No. 247 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the determination of chloramphenicol residues in aquatic products sample preparation and analysis of radiation receptor (Charm ��) method. This standard applies to chloramphenicol residues in aquatic products screening assays. | SN/T 1966-2007: Determination of chloramphenicol residues in aquatic product. Radio-receptor assay method
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of chloramphenicol residues in aquatic product.Radio-receptor assay method
Exit inspection and quarantine industry standard book People's Republic of China
Detection of chloramphenicol residues in aquatic products
Radioreceptor assay
Posted 2007-08-06
2008-03-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Guangdong CIQ.
The main drafters of this standard. phase Dapeng Chen snow, Zhanglin Tian, Chen Yanqin, Liang Xiyang, Luo Huiming, £ - Zhuang Yilin.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Detection of chloramphenicol residues in aquatic products
Radioreceptor assay
1 Scope
This standard specifies the determination of chloramphenicol residues in aquatic products for sample preparation and radioreceptor assay (CharmⅡ) method.
This standard applies to the screening assays of chloramphenicol residues in aquatic products.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Sample preparation and preservation
3.1 Preparation of the sample
All samples taken from a representative sample of not less than 500g, high-speed tissue masher mashing even the crushed sample is thoroughly mixed
uniform. Loaded into a clean container, sealed and do identity.
In the sample preparation process, should prevent sample contamination or the occurrence of the change of residue content. Samples for determining the number of bacteria can not exceed
Over 106/g.
3.2 Sample preservation
The sample was stored frozen at -18 ℃ below.
4 Determination
4.1 Principle
Based assay is a competitive receptor immunoreactivity. After chloramphenicol residues in samples were extracted, and [3H] labeled chloramphenicol competition fine
Chloramphenicol in bacterial cells specific receptors, chloramphenicol higher drug content in the sample, the more sites compete for binding, [3H] labeled chloramphenicol
Biotin-binding sites of the less. After the reaction at a certain temperature, centrifuged, clear the [3H] labeled chloramphenicol unbound, scintillation fluid added after use
LSC measurement sample of [3H] content cpm value (countperminute, that is, the number of pulses per minute). The lower the cpm value,
The higher the sample chloramphenicol residues.
4.2 Reagents and materials
4.2.1 Water
GB/T 6682 provided a water.
The new kit batch performance monitoring shall be carried out before use.
1) CharmⅡ competitive receptor binding organization chloramphenicol test kit is provided by the trade name of the company's products Charm. This information is given
The convenience of the user of this standard does not imply endorsement of the product. If other equivalent product has the same effect, you can use these equivalent
product.
4.2.2.1 Competitive binding reagent. should be stored at -15 ℃ or less, up to 6h place at room temperature (15 ℃ ~ 25 ℃) under.
4.2.2.2 [3H] labeled chloramphenicol. should be stored at -15 ℃ or less, up to 6h place at room temperature (15 ℃ ~ 25 ℃) under.
|