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Screening method of fumonisin B1 residues bin foodstuff for import and export. Enzyme linked immunosorbent assay method
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SN/T 1958-2007
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Basic data | Standard ID | SN/T 1958-2007 (SN/T1958-2007) | | Description (Translated English) | Screening method of fumonisin B1 residues bin foodstuff for import and export. Enzyme linked immunosorbent assay method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.050 | | Word Count Estimation | 17,179 | | Date of Issue | 2007-08-06 | | Date of Implementation | 2008-03-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | Accredidation-Technology-Letter [2015] No. 247; National Health and Family Planning Commission Bulletin No. 11 of 2016 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the food fumonisins B Residual ELISA method. This standard applies to corn, rice, yellow rice, oats, barley and peanut fumonisins B residue screening tests. | SN/T 1958-2007: Screening method of fumonisin B1 residues bin foodstuff for import and export. Enzyme linked immunosorbent assay method
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Screening method of fumonisin B1 residues bin foodstuff for import and export.Enzyme linked immunosorbent assay method
Exit inspection and quarantine industry standard book People's Republic of China
Import and export food fumonisins B1 Residue Detection
Enzyme-linked immunosorbent assay
Posted 2007-08-06
2008-03-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
The Standard Appendix A, Appendix B and Appendix C are informative appendices.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Tianjin People's Republic of China Exit Inspection and Quarantine, Tianjin University of Science and Technology.
The main drafters of this Standard. Kit Cheng, Wang Shuo, Zhang Hongwei, Yao Xia, Wei Yadong Liu, Zhang Yan, Zhang Jun?.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Import and export food fumonisins B1 Residue Detection
Enzyme-linked immunosorbent assay
1 Scope
This standard specifies the food fumonisins B1 Residues ELISA method.
This standard applies to corn, rice, yellow rice, oats, barley and peanut fumonisins B1 residues screening tests.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Determination
3.1 Method summary
Fumonisins B1 samples were extracted with methanol/water, detected using a competitive enzyme-linked immunosorbent assay.
3.2 Reagents and materials
Unless otherwise requested, all reagents were of analytical grade water as a water GB/T 6682 regulations.
3.2.1 fumonisin B1 ELISA assay kit 1) (see Appendix A).
1) fumonisin B1 ELISA kit provided by the Tianjin Biochip Technology Co., Ltd. under the trade name product. This gives
Information is for the convenience of users of this standard does not imply endorsement of the product. If other equivalent product has the same effect, you can use this
Some equivalent.
3.2.2 methanol.
3.2.3 disodium hydrogen phosphate (Na2HPO4 · 12H2O).
3.2.4 sodium dihydrogen phosphate (NaH2PO4 · 2H2O).
3.2.5 sodium chloride.
3.2.6 masking agent Diluent. a masking agent. phosphate buffer (1.20, by volume), and diluting at the time of use.
3.2.7 Ⅰ solution. methanol. masking agent dilutions (1.10, by volume).
3.2.8 fumonisin B1 standard (English name. fumonisinB1, CAS Code. 116355-83-0). not less than 99.5% purity.
3.2.9 Standard stock solution. formulated as 10mg/L stock solution of methanol, -20 ℃ preservation, valid for three months.
Cryopreservation agents, stand at room temperature should not exceed 4h, when used, should each solution to reach room temperature.
3.3 instruments and equipment
3.3.1 grinder.
3.3.2 scales (accurate to 0.001g).
3.3.3 vortex mixer.
3.3.4 Gas bath shaker.
3.3.5 microplate reader (measurement wavelength 450nm).
3.3.6 washer.
3.3.7 Single-channel micro pipette. 20μL, 100μL, 200μL.
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