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SN/T 1956-2007: Determination of diethylstibestrol residues in meat and meat product. Enzyme linked immunosorbent assay
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1956-2007 | English | 519 |
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Determination of diethylstibestrol residues in meat and meat product. Enzyme linked immunosorbent assay
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SN/T 1956-2007
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Basic data | Standard ID | SN/T 1956-2007 (SN/T1956-2007) | | Description (Translated English) | Determination of diethylstibestrol residues in meat and meat product. Enzyme linked immunosorbent assay | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.050 | | Word Count Estimation | 16,143 | | Date of Issue | 2007-08-06 | | Date of Implementation | 2008-03-01 | | Regulation (derived from) | Accredidation-Technology-Letter [2015] No. 247 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the meat and meat diethylstilbestrol residues in enzyme-linked immunoassay. This standard applies to chicken, pork, beef, lamb, pork pie in the detection of diethylstilbestrol residues. | SN/T 1956-2007: Determination of diethylstibestrol residues in meat and meat product. Enzyme linked immunosorbent assay
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of diethylstibestrol residues in meat and meat product.Enzyme linked immunosorbent assay
Exit inspection and quarantine industry standard book People's Republic of China
Diethylstilbestrol residues in meat and meat products Detection
ELISA
Posted 2007-08-06
2008-03-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
The Standard Appendix A and Appendix B are informative.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Tianjin People's Republic of China Exit Inspection and Quarantine.
The main drafters of this standard. Sun Li, Kit Cheng, Tang Dan Zhou, Wei Yadong, Zhang Hongwei.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Diethylstilbestrol residues in meat and meat products Detection
ELISA
1 Scope
This standard specifies the method of enzyme-linked immunosorbent assay meat and meat products diethylstilbestrol residues.
This standard applies to chicken, pork, beef, lamb, pork pie diethylstilbestrol residues detected.
2 Sample preparation and preservation
2.1 Preparation of the sample
Remove all the samples taken from a representative sample of about 1kg, fully crush, mix, use quartering the sample into two equal parts, installed
Into a clean container, sealed and do identity.
Save 2.2 sample
Sample was placed at -20 ℃ ~ -18 ℃ conditions of preservation.
3 Determination
3.1 Method summary
Determination of the basis of this method is a competitive enzyme immunoassay. Diethylstilbestrol and diethylstilbestrol enzyme markers common competition diethylstilbestrol antibody
Binding site, measuring the absorbance value pore solution using a microplate reader, and absorbance is inversely proportional to the concentration of diethylstilbestrol, according to a calibration curve plotted
Quantitative calculation.
3.2 Reagents and materials
Unless otherwise specified, the use of chemical reagents were of analytical grade or higher level. Water re-distilled water.
3.2.1 diethylstilbestrol quantitative enzyme-linked immunosorbent assay kit (see Appendix A).
3.2.2 tert-butyl methyl ether. chromatography.
3.2.3 petroleum ether.
3.2.4 dichloromethane.
3.2.5 phosphoric acid.
3.2.6 hydrochloric acid.
3.2.7 Methanol. chromatographically pure.
3.2.8 disodium hydrogen phosphate (Na2HPO4 · 2H2O).
3.2.9 sodium dihydrogen phosphate (NaH2PO4 · H2O).
3.2.10 sodium chloride.
3.2.11 Tris.
3.2.12 phosphate buffer (67mmol/L, pH = 7.2). Weigh 9.61g disodium hydrogen phosphate, 1.79g sodium dihydrogen phosphate and 8.7g
Sodium chloride is dissolved in water and dilute to 1000mL.
3.2.13 Tris buffer (20mmol/L, pH = 8.5). Weigh 2.42g Tris was dissolved in 700mL of water, plus
Into 200mL of methanol, adjusting 5mol/L hydrochloric acid to pH 8.5, add water to 1000mL.
3.2.14 washed with phosphate buffer (20mmol/L, pH = 7.2). Weigh 2.85g disodium hydrogen phosphate, 0.55 g sodium dihydrogen phosphate,
9g 1mL sodium chloride and Tween 20, was dissolved in water and dilute to 1000mL.
3.2.15 sodium hydroxide solution (1mol/L). Weigh 40g of sodium hydroxide dissolved in 1000mL of water.
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