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Detection of Brucella in food from animal for import and export. Part 2: PCR method
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SN/T 1942.2-2007
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Basic data | Standard ID | SN/T 1942.2-2007 (SN/T1942.2-2007) | | Description (Translated English) | Detection of Brucella in food from animal for import and export. Part 2: PCR method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.050; 07.100.30 | | Word Count Estimation | 10,160 | | Date of Issue | 2007-08-06 | | Date of Implementation | 2008-03-01 | | Quoted Standard | GB 19489; SN/T 1942.1; WS/T 230-2002 | | Regulation (derived from) | Accredidation-Technology-Letter [2015] No. 247 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the import and export of foodstuffs of animal origin Brucella PCR testing methods. This standard applies to import foodstuffs of animal origin Brucella rapid test. | SN/T 1942.2-2007: Detection of Brucella in food from animal for import and export. Part 2: PCR method
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Detection of Brucella in food from animal for import and export.Part 2. PCR method
Exit inspection and quarantine industry standard book People's Republic of China
Import and export food of animal origin in Brucella
Testing method
Part 2. PCR testing methods
Posted 2007-08-06
2008-03-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 1942-2007 "import and export food of animal origin in Brucella test method" is divided into two parts.
--- Part 1. Separation and counting method;
Part --- Article 2. PCR test.
This section SN/T 1942 Part 2.
This section of the Appendices A and B are normative appendix.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section was drafted by. People's Republic of China Liaoning Entry-Exit-Entry Inspection and Heilongjiang People's Republic of China
Quarantine Bureau.
The main drafters of this section. Woo, Moon Zheng, Jia
, Lisu Long, LI Zhen-Rong, Hu Zhuanwei.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Import and export food of animal origin in Brucella
Testing method
Part 2. PCR testing methods
1 Scope
SN/T 1942 This section provides the import and export of foodstuffs of animal origin Brucella PCR test.
This section applies to the import and export food of animal origin in rapid testing of the genus Brucella.
2 Normative references
The following documents contain provisions which, through reference SN/T 1942 to the present, constitute provisions of this section. For dated reference documents
Member, all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouraged to reach under this section
Parties to research agreement to use the latest versions of these documents. For undated reference documents, the latest versions apply to this
section.
GB 19489 General requirements for laboratory biosafety
SN/T 1942.1 and export foodstuffs of animal origin Brucella test methods - Part 1. Separation and Counting
Its application Technology WS/T 230-2002 clinical diagnosis Polymerase
3 Biosecurity
In order to protect the safety of laboratory personnel, the staff shall be qualified to detect Brucella, all cultures and waste should be careful
Disposal. And in accordance with the relevant provisions of GB 19489.
4 anti-pollution measures
Referring WS/T 230-2002 in Chapter 6, the prevention and control of pollution.
5 terms, definitions and abbreviations
5.1 Terms and Definitions
The following terms and definitions apply to SN/T 1942 of this part.
5.1.1
Brucella is a class of Gram-negative bacilli short ball, biodegradable sugars such as glucose, less acid production, no power, no spores, peroxide
Catalase positive.
5.1.2
Use two (usually a length of 15 to 25 nucleotides) as a reaction oligodeoxynucleotide primers, these two primer sequences should not
Occurred complementary roles and specific sites can be tested on the two strands of DNA as a template occurs complementary respectively. The reaction solution containing magnesium include
Reaction buffer ions, four kinds of deoxynucleotide triphosphates (of dNTP), a template DNA, primers and thermostable DNA polymerase composition. in
DNA polymerase catalyzed by repeated changes of DNA denaturation, annealing and extension dozens of cycles obtained between two complementary sites
Large number of copies of the DNA fragment.
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