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| SN/T 1941.3-2007 | English | 399 |
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Detection of lactic acid bacteria in food for import and export. Part 3: Lactobacillus PCR method
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SN/T 1941.3-2007
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Basic data | Standard ID | SN/T 1941.3-2007 (SN/T1941.3-2007) | | Description (Translated English) | Detection of lactic acid bacteria in food for import and export. Part 3: Lactobacillus PCR method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.050; 07.100.30 | | Word Count Estimation | 10,185 | | Date of Issue | 2007-08-06 | | Date of Implementation | 2008-03-01 | | Quoted Standard | GB 19489; SN/T 1941.1; WS/T 230-2002 | | Regulation (derived from) | Accredidation-Technology-Letter [2015] No. 247 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies test methods PCR lactobacilli. This standard applies to natural or adding lactic acid bacteria in food and raw materials Determination of lactic acid bacteria. | SN/T 1941.3-2007: Detection of lactic acid bacteria in food for import and export. Part 3: Lactobacillus PCR method
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Detection of lactic acid bacteria in food for import and export.Part 3. Lactobacillus PCR method
Exit inspection and quarantine industry standard book People's Republic of China
Lactic acid bacteria in food import and export inspection methods
Part 3. Lactobacillus PCR method
Posted 2007-08-06
2008-03-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 1941 "lactic acid bacteria in food import and export inspection method" is divided into three parts.
--- Part 1. Separation and counting method;
--- Part 2. PetrifilmTM test plate method;
--- Part 3. Lactobacillus PCR method.
This section SN/T 1941 Part 3.
This section of the Appendices A and B are normative appendix.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section was drafted by. People's Republic of China Liaoning Province Exit Inspection and Quarantine.
The main drafters of this section. Woo, Moon Zheng, Li Ye, Huzhuan Wei, Li Zhenrong.
The first part of the Department of Inspection and Quarantine issued by industry standards.
Lactic acid bacteria in food import and export inspection methods
Part 3. Lactobacillus PCR method
1 Scope
SN/T 1941 This section provides PCR testing methods lactobacilli.
This section applies to natural or added food and feed Determination of lactic acid bacteria of lactic acid bacteria.
2 Normative references
The following documents contain provisions which, through reference SN/T 1941 to the present, constitute provisions of this section. For dated reference documents
Member, all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouraged to reach under this section
Parties to research agreement to use the latest versions of these documents. For undated reference documents, the latest versions apply to this
section.
GB 19489 General requirements for laboratory biosafety
SN/T 1941.1 lactic acid bacteria in food import and export inspection method - Part 1. Separation and Counting
Its application Technology WS/T 230-2002 clinical diagnosis Polymerase
3 Biosecurity
In order to protect the safety of laboratory personnel, the staff shall be qualified to detect lactic acid bacteria, all cultures and waste should be careful
Disposal. And in accordance with the relevant provisions of GB 19489.
4 anti-pollution measures
Referring WS/T 230-2002 in Chapter 6, the prevention and control of pollution.
5 terms, definitions and abbreviations
5.1 Terms and Definitions
The following terms and definitions apply to SN/T 1941 to the present section.
5.1.1
Lactobacillus is a class of Gram-positive bacilli without spores elongate, can break down lactose, glucose or lactic acid pollution, obligate anaerobic,
Anaerobic or facultative anaerobic, mostly without power, catalase negative.
5.1.2
With two (usually a length of 15 to 25 nucleotides) as a reaction oligodeoxynucleotide primers and the template DNA strand test
Specific sites are complementary to happen. In a suitable catalytic reaction liquid DNA polymerase by DNA denaturation, annealing and extension dozens
Circulating large number of copies is obtained between two complementary DNA fragments sites. The reaction liquid comprises a reaction buffer containing magnesium ions, four kinds deoxy
Triphosphates (dNTP), a template DNA, primers and thermostable DNA polymerase composition.
5.1.3
Application of chemical synthesis known to be one pair of amplification of gene fragments complementary to the DNA sequence on both sides of oligonucleotides as PCR amplification
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