| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1907-2007 | English | 279 |
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Protocol of the PCR for mycobacterium paratuberculosis
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SN/T 1907-2007
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Basic data | Standard ID | SN/T 1907-2007 (SN/T1907-2007) | | Description (Translated English) | Protocol of the PCR for mycobacterium paratuberculosis | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 65.020.30 | | Word Count Estimation | 7,761 | | Date of Issue | 2007-05-23 | | Date of Implementation | 2007-12-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | ?AQSIQ-Inspection [2010] 582 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the analysis of Mycobacterium paratuberculosis PCR detection method. Feces and milk analysis of Mycobacterium paratuberculosis detection of sheep and other animals, anti- wrinkle, the standard for cattle. | SN/T 1907-2007: Protocol of the PCR for mycobacterium paratuberculosis---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of the PCR for mycobacterium paratuberculosis
Exit inspection and quarantine industry standard book People's Republic of China
Mycobacterium paratuberculosis PCR Detection Technology
Practice
Posted 2007-05-23
2007-12-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
The Standard Appendix A and Appendix B are informative.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Shanxi People's Republic of China Exit Inspection and Quarantine.
The main drafters of this standard. Gong Hongxia, Hui Feng Lian, even GY, strong Gong, Wu Haijun, Lei Yuping, Li Weihua, Zhangzu Wei.
This is the first issue of inspection and quarantine industry standards.
Mycobacterium paratuberculosis PCR Detection Technology
Practice
1 Scope
This standard specifies the Mycobacterium paratuberculosis PCR detection method.
This standard applies to cattle, sheep and other ruminant animals, manure and milk Mycobacterium paratuberculosis detection.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
Laboratory use specifications and test methods (. 1987 eqv ISO 3696) GB/T 6682 Analysis
3 Reagents and materials
3.1 The main instruments
3.1.1 PCR thermal cycler.
3.1.2 scales.
3.1.3 clean bench.
3.1.4 Sterilization pot.
3.1.5 High-speed refrigerated centrifuge.
3.1.6 low temperature freezer, refrigeration refrigerator.
3.1.7 test water preparation device.
3.1.8 high temperature oven.
3.1.9 Vortex.
3.1.10 gel imaging analysis system.
3.1.11 constant temperature water bath oscillator.
3.1.12 magnetic stirrer.
3.1.13 micropipette.
3.2 Reagents
3.2.1 Unless otherwise specified, all reagents were analytical grade or the use of chemical reagents.
3.2.2 Test water. should comply with GB/T 6682 in a water specifications. The reagent formulation in Appendix A.
4 detection step
4.1 Preparation of the sample
4.1.1 standard strains. Mycobacterium paratuberculosis by AQSIQ designated units.
4.1.2 stool. Weigh fresh feces 1g, plus 3mLPBS (pH7.4 See Chapter A.5), mixing, placing 30min or 300r/min
5min centrifugation at room temperature, and the supernatant, at room temperature for 30min or 300r/min centrifugal 5min at room temperature once again, and the supernatant, 12000r/min
RT centrifugal 15min, discard supernatant, 400μLTE.
4.1.3 milk samples. Take 1mL aseptic milk and 10μLTritonX-100, mixing, 12000r/min at room temperature centrifugal 15min, supernatant was discarded
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