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| SN/T 1905-2007 | English | 399 |
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Bovine viral diarrhea / mucosal disease reverse transcription polymerase chain reaction operating procedures
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SN/T 1905-2007
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Basic data | Standard ID | SN/T 1905-2007 (SN/T1905-2007) | | Description (Translated English) | Bovine viral diarrhea / mucosal disease reverse transcription polymerase chain reaction operating procedures | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 10,122 | | Date of Issue | 2007-05-23 | | Date of Implementation | 2007-12-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | ?AQSIQ notice published batches of 2015 No. 103 entry-exit inspection and quarantine industry standard | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the bovine viral diarrhea/mucosal disease virus reverse transcription polymerase chain reaction detection methods. This standard applies to bovine viral diarrhea/mucosal disease virus is detected. | SN/T 1905-2007: Bovine viral diarrhea / mucosal disease reverse transcription polymerase chain reaction operating procedures
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Bovine viral diarrhea/mucosal disease reverse transcription polymerase chain reaction operating procedures
Exit inspection and quarantine industry standard book People's Republic of China
Bovine viral diarrhea/mucosal disease reverse transcription polymerase chain
The reaction procedures
Posted 2007-05-23
2007-12-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard is a normative appendix, Appendix B is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Jilin People's Republic of China Exit Inspection and Quarantine, Shenzhen Co., Pittsburgh-based bio-engineering.
The main drafters of this standard. Spacing Form, Wang Zhenguo, Liu Jinhua, Bangladesh on the increase, as Rui Xiao, Jian-Ping Shi, Mou Jun, Pang Chun Kui, Yang Wei, Lai Shaomei.
This is the first issue of inspection and quarantine industry standards.
Bovine viral diarrhea/mucosal disease reverse transcription polymerase chain
The reaction procedures
1 Scope
This standard specifies the bovine viral diarrhea/mucosal disease virus reverse transcriptase polymerase chain reaction detection method.
This standard applies to bovine viral diarrhea/mucosal disease virus detection.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
Laboratory use specifications and test methods GB/T 6682 Analysis
Principle 3
According to bovine viral diarrhea/mucosal disease virus genome 5 'end of the nucleotide sequence of the most conservative of the non-coding region were designed and synthesized a pair of specific
Primers by reverse transcription polymerase chain reaction using one-step and two-step method were amplified from various samples of bovine viral diarrhea/mucosal
Specific fragments of the virus, the fragment can be used viral diarrhea/mucosal disease virus genotyping and can be distinguished from other pestiviruses
member.
4 Equipment and Reagents
4.1 The main instruments and equipment
4.1.1 PCR amplification.
4.1.2 low-speed centrifuge, high-speed micro-centrifuge.
4.1.3 electrophoresis, electrophoresis tank.
4.1.4 gel imaging analysis system.
4.1.5 fridge.
4.1.6 clean bench.
4.1.7 tissue homogenizer.
4.1.8 carbon dioxide incubator.
4.2 Reagents and Consumables
4.2.1 Water. The water should be consistent with GB/T 6682 in the three water (triple-distilled water) specifications. Water used for PCR (in particular nucleic acid extraction) of use
DEPC-treated to remove RNA enzyme, method of preparation, see Section A. Chapter 1.
4.2.2 nutrient solution. preparation methods see Section A. 2.
4.2.3 maintain the solution. preparation methods, see Section A. 3.
4.2.4 cell dispersion. preparation methods see Section A. 4.
4.2.5 Chemicals. All chemicals were of analytical grade. Catrimox-14 (Cat-14), guanidine thiocyanate, phenol, chloroform, lemon
Sodium n-butoxide, sodium acetate, ethanol, isopropanol (-20 ℃ precooled).
4.2.6 Other reagents. RT-PCRBeads (reverse transcriptase) or AMV reverse transcriptase (5U/μL), T Rao q DNA polymerase (5U /
μL), dNTPs (each concentration was 10mmol/L), RNasin (40U/μL), MgCl2 (15mmol/L), agarose (electrophoresis grade),
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