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SN/T 1900-2007: Identification of Cephalosporium maydis Samra, Sabet et Hingorani
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1900-2007 | English | 229 |
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Identification of Cephalosporium maydis Samra, Sabet et Hingorani
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SN/T 1900-2007
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Basic data | Standard ID | SN/T 1900-2007 (SN/T1900-2007) | | Description (Translated English) | Identification of Cephalosporium maydis Samra, Sabet et Hingorani | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020 | | Word Count Estimation | 7,757 | | Date of Issue | 2007-05-23 | | Date of Implementation | 2007-12-01 | | Regulation (derived from) | National-accreditation-Science [2011] 63 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the entry of corn seeds and corn on the cephalosporin entrainment enzyme quarantine and identification methods. This standard applies to late blight from the corn occur in all countries and regions of the corn for processing and reproduction of the quarantine. | SN/T 1900-2007: Identification of Cephalosporium maydis Samra, Sabet et Hingorani---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Identification of Cephalosporium maydis Samra, Sabet et Hingorani
Exit inspection and quarantine industry standard book People's Republic of China
Corn quarantine and identification of late blight pathogen
Posted 2007-05-23
2007-12-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard and Appendix B is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. China Inspection and Quarantine Science Research Institute, People's Republic of China Liaoning Province Exit Inspection and Quarantine.
Drafters of this standard. Yan Jin, Wu Shan goods, Liu Wei.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Corn quarantine and identification of late blight pathogen
1 Scope
This standard applies to late blight of corn from all the quarantine occurred for corn processing and reproduction of countries and regions.
Principle 2
2.1 taxonomic status
English name. LateWiltofMaize
2.2 Identification of principle
This fungus can spread by seed, seed-borne 11%. In addition, this fungus can also survive in the form of sclerotia in plant debris. This fungus
Facultative parasites. Thus, the pathogen can be isolated from the seeds and plant residues and morphological identification.
3 apparatus and appliances
3.1 Instrument
Stereo microscope, optical microscope (with oil immersion and micrometer), the general balance (inductance 0.1g), clean bench, light incubator
Or light training aircraft, near ultraviolet lamps (based on culture incubator or frame size), autoclave.
3.2 Appliances
Hand-held magnifying glass, paper, petri dishes, beakers, flasks, tweezers, scissors, slides, cover slips, measuring pipette, alcohol lamp.
4 Reagents and culture medium
4.1 Reagents
Potato, dextrose, agar, sterile water, 1.25% sodium hypochlorite, 75% alcohol.
4.2 Medium
Potato dextrose medium (PDA).
5 identification methods
5.1 Symptom Check
Infested seed surface asymptomatic. Infested stems dry, sunken, hollow, vascular bundle becomes reddish brown.
5.2 Moisture culture
5.2.1 Seed Moisturizer culture
Detected by freezing filter paper method. Each sample taken 400 seeds with 1.25% sodium hypochlorite (NaOCl) solution surface sterilized
After 3min ~ 4min, on the mat three sterilization wet filter paper in petri dishes, each dish 10, at room temperature 20 ℃ ~ 22 ℃ placed after 24h, in
-20 ℃ frozen 24h, then go to 25 ℃ incubator or shelf culture cultured 7d, according to near-ultraviolet light and dark alternately each 12h.
5.2.2 plant residues moisturizing culture
Plant residues 1min immersed in 75% alcohol solution, washed with sterile water through pads placed on the three-moistened filter paper in a petri dish, in
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