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| SN/T 1819-2006 | English | 239 |
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Import and export latex medical gloves protein determination. Improved the method of Lowry
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SN/T 1819-2006
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PDF similar to SN/T 1819-2006
Standard similar to SN/T 1819-2006 YY/T 0243 HAD 002/01 HG/T 2584 Basic data | Standard ID | SN/T 1819-2006 (SN/T1819-2006) | | Description (Translated English) | Import and export latex medical gloves protein determination. Improved the method of Lowry | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | G45 | | Classification of International Standard | 11.140; 83.140 | | Word Count Estimation | 6,638 | | Date of Issue | 2006-08-28 | | Date of Implementation | 2007-03-01 | | Quoted Standard | GB/T 6682 | | Adopted Standard | ASTM D5712-1999, NEQ | | Regulation (derived from) | ?National-accreditation-Science [2010] 50; Accreditation-Science [2014] 263; 2014- inspection and quarantine industry standard review conclusions | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the protein content of latex medical gloves in the method. This standard applies to medical gloves in latex protein content. This standard latex protein content range: 7��g/dm2 ~ 200��g/dm2. | SN/T 1819-2006: Import and export latex medical gloves protein determination. Improved the method of Lowry
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Import and export latex medical gloves protein determination.Improved the method of Lowry
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
Determination of latex protein in medical gloves for import and export
Released on.2006-08-28
2007-03-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard refers to ASTM D5712-1999 "Determination of water-soluble proteins in natural rubber and its products - improved Lowry method
Formulation.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Ningbo Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Tong Lubo, Ge Xuanning.
This standard is the first industry standard for entry-exit inspection and quarantine.
Determination of latex protein in medical gloves for import and export
1 Scope
This standard specifies the method for determining the protein content of latex in medical gloves.
This standard applies to the determination of latex protein content in medical gloves.
The range of determination of latex protein content in this standard. 7μg/dm
2～200μg/dm
2.
2 Normative references
The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all
Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study
Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard.
GB/T 6682 Analytical laboratory water specifications and test methods
3 Method summary
Latex protein in medical gloves and natural rubber products can be extracted from the buffer solution and precipitated to remove soluble interferences.
The Lowry method measures the absorbance at a wavelength of 750 nm from a spectrophotometer.
4 Terms and definitions
The following terms and definitions apply to this standard.
4.1
The sodium hydroxide capacity (mL) used to dissolve the sample to extract the precipitate and the sodium hydroxide used to decompose the standard ovalbumin extract
The ratio of capacity (mL).
Note. For example, 5mL test extract, precipitated and dissolved with 1.25mL sodium hydroxide, standard egg protein is dissolved with sodium hydroxide, and its dissolved volume is also
At 1.25 mL, the dilution rate of the test extract is the same as the dilution rate of the standard curve.
4.2
Latex proteins and polypeptides from natural rubber and its products.
4.3
Quantitative detection limit LOQ
The lowest protein content that can be quantitatively measured and produces accurate results. The LOQ is set to a standard deviation of 10 times.
5 instruments
5.1 Centrifuge.
5.2 Spectrophotometer.
5.3 Vortex mixer.
5.4 Micro-adjustable pipette (0.2mL ~ 5.0mL), volumetric flask (50mL, 100mL).
5.5 Polypropylene test tubes. capacities of 50 mL and 10 mL.
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