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SN/T 1812-2006: Identification of Tilletia fusca Ellis and Everhart, Tilletia sphaerococca (Wallroth) Fischer v Waldheim and Tilletia lolii Auerswald ex Winter
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Identification of Tilletia fusca Ellis and Everhart, Tilletia sphaerococca (Wallroth) Fischer v Waldheim and Tilletia lolii Auerswald ex Winter
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SN/T 1812-2006
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PDF similar to SN/T 1812-2006
Basic data | Standard ID | SN/T 1812-2006 (SN/T1812-2006) | | Description (Translated English) | Identification of Tilletia fusca Ellis and Everhart, Tilletia sphaerococca (Wallroth) Fischer v Waldheim and Tilletia lolii Auerswald ex Winter | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020 | | Word Count Estimation | 8,851 | | Date of Issue | 2006-08-28 | | Date of Implementation | 2007-03-01 | | Quoted Standard | GB/T 18085-2000 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the entry plant quarantine grasses fishy Ustilago (Tilletia fusca), bentgrass Tilletia fungus (Tilletia sphaero-cocca), ryegrass Tilletia fungus (Tilletia lolii) proposal identification. This standard applies to Bromus, Festuca, Poa and other grasses fungus Tilletia fishy host grasses grasses in Ustilago, Agrostis grass in bentgrass and black fungus Tilletia grasses in ryegrass straw genus Tilletia quarantine and identification of bacteria. | SN/T 1812-2006: Identification of Tilletia fusca Ellis and Everhart, Tilletia sphaerococca (Wallroth) Fischer v Waldheim and Tilletia lolii Auerswald ex Winter
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Identification of Tilletia fusca Ellis and Everhart, Tilletia sphaerococca (Wallroth) Fischer v Waldheim and Tilletia lolii Auerswald ex Winter
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
Black grass, black stalk, ryegrass
Phytosanitary identification method
Released on.2006-08-28
2007-03-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
Appendix A, Appendix B and Appendix C of this standard are informative annexes.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Tianjin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Luo Jiafeng, Huang Guoming, Cui Tiejun, Liu Yueting, Liao Fang.
This standard is the first industry standard for entry-exit inspection and quarantine.
Black grass, black stalk, ryegrass
Phytosanitary identification method
1 Scope
This standard is applicable to the black grasses of the genus Brome, the fescue, the bluegrass and other grasses of the genus Heliconia, and the genus
Quarantine and identification of ryegrass sphaeroides from the genus Sphaerotheca fuliginea and ryegrass in the grass species.
2 Normative references
The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all
Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study
Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard.
GB/T 18085-2000 Quarantine and identification method for wheat dwarf mites
3 Principle
The infected plants are in real time, and the seeds are invaded by the pathogen teliospores to form bacillary dysentery. The germs spread with the seeds over long distances. Pathogen teliospores
The characteristics of learning and the physiology of germination are the main basis for the identification of these three sphagnum. See Appendix A, Appendix B and the main distribution areas.
Appendix C.
4 instruments
Multi-function microscope, stereo microscope, low-speed centrifuge, reciprocating oscillator, vortex oscillator, dry heat sterilizer, autoclave, general
Tongping (sensing 1/100g), biological safety cabinet or ultra-clean platform, biological incubator (with light, low temperature can be adjusted to 5 °C), adjustable micro-sampler
(5μL ~.200μL), triangular flask (250mL), centrifuge tube, glass slide (length × width is 25.4mm × 76.2mm, thickness 0.8mm), cover glass
Sheet (18 mm × 18 mm), Parafilm film or aluminum platinum paper, white porcelain plate, Eppendorf tube (1.5 mL), Petri dish.
5 pharmaceutical reagents
Sodium hypochlorite solution or bleaching powder (chemically pure), Tween-20, Schier's floating agent (see GB/T 18085-2000)
Appendix A. Preparation method of Schir's float carrier), agar powder, non-fluorescent microscope objective lens soaking oil (Nd=1.516), sealing tablets.
6 Laboratory tests
6.1 Sample preparation
After the sample is received, the original sample is thoroughly mixed in the sample bag in the sample preparation chamber to make an average sample. Weigh from the average sample
50 g was used as a test sample.
6.2 Washing inspection
6.2.1 Washing and centrifugation
Pour the test sample into a sterile flask, add 100 mL of distilled water, add Tween-201 drops to 2 drops, use aluminum platinum paper or Parafilm film.
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