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Protocol of indirect dot enzyme-linked immunosorbent assay for the detection of porcine epidemic diarrhea
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SN/T 1699.3-2006
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Basic data | Standard ID | SN/T 1699.3-2006 (SN/T1699.3-2006) | | Description (Translated English) | Protocol of indirect dot enzyme-linked immunosorbent assay for the detection of porcine epidemic diarrhea | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 9,983 | | Date of Issue | 2006-01-26 | | Date of Implementation | 2006-08-16 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | Industry Standard Notice 2006 No. 4 (total of 76) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the porcine epidemic diarrhea indirect dot ELISA detection method. This standard applies to porcine epidemic diarrhea virus antibodies. Can be used for this disease quarantine, surveillance and epidemiological investigation. | SN/T 1699.3-2006: Protocol of indirect dot enzyme-linked immunosorbent assay for the detection of porcine epidemic diarrhea
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Protocol of indirect dot enzyme-linked immunosorbent assay for the detection of porcine epidemic diarrhea
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
Porcine epidemic diarrhea indirect spot enzyme-linked immunosorbent assay
Adsorption test protocol
Released on.2006-01-26
Implementation of.2006-08-16
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
SN/T 1699 is divided into three parts.
--- Swine epidemic diarrhea trace serum neutralization test procedures;
--- Swine epidemic diarrhea virus direct immunofluorescence test procedures;
--- Indirect spot enzyme-linked immunosorbent assay protocol for porcine epidemic diarrhea.
This part is the third part of SN/T 1699.
Appendix A of this part is a normative appendix, and Appendix B is an informative appendix.
This part is proposed and managed by the National Certification and Accreditation Administration.
This section drafted by. Guangdong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this section. Chen Ru, Luo Qiong, Lin Zhixiong, Liu Linlin.
This section is the first industry standard for entry-exit inspection and quarantine.
Introduction
Porcine epidemic diarrhea (PEDV) is a type of porcine epidemic diarrhea virus (PEDV)
Porcine intestinal infectious diseases characterized by diarrhea, vomiting and dehydration, and clinical manifestations are very similar to porcine transmissible gastroenteritis (TGE). In 1978, than
For the first time, the pathogen was confirmed to be a coronavirus. Due to the lack of stable vaccine production, the disease has become a developed country in Europe and Asia.
It is the key research object of pig disease prevention and control in the region, and is listed in the list of infectious diseases in China.
PED is not significantly different from TGE in terms of epidemiology, clinical manifestations and viral morphology. The diagnosis is mainly based on specific serology and
Molecular biology methods.
The indirect method of dot-enzyme-linked immunosorbent assay (Dot-ELISA) can rapidly detect the presence of PEDV in the serum of infected animals.
Body, especially suitable for primary screening of large samples.
Indirect spot enzyme-linked immunosorbent assay protocol for porcine epidemic diarrhea
1 Scope
This part of SN/T 1699 specifies an indirect spot enzyme-linked immunosorbent assay for detection of porcine epidemic diarrhea.
This section applies to the detection of antibodies to porcine epidemic diarrhea virus. It can also be used for quarantine, epidemic surveillance and epidemiological investigation of this disease.
2 Normative references
The provisions in the following documents become the provisions of this part by reference to this part of SN/T 1699. Quotations with dated
, all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouragement is achieved under this section
The parties to the agreement study whether the latest versions of these documents can be used. For undated references, the latest edition applies to this
section.
GB/T 6682 Analytical laboratory water specifications and test methods
3 Principle
The dot-enzyme-linked immunosorbent assay is an immunoenzymatic labeling technique, the principle and procedure of which are compared with conventional enzyme-linked immunosorbent assay (ELISA).
Basically the same, the difference is that the solid phase adsorption carrier is replaced by a solid phase matrix membrane for adsorption of antigen or antibody, and insoluble
The hydrogen donor was used as a chromogenic substrate, and as a result, it was judged by the occurrence of colored spots on the matrix film.
4 Abbreviations
The following abbreviations apply to this part of SN/T 1699.
4.1
Spot enzyme-linked immunosorbent assay.
4.2
HRP-SPA
Horseradish peroxidase-labeled staphylococcal protein A.
5 Equipment and equipment
5.1 Shaker.
5.2 Constant temperature incubator.
5.3 Adjustable single-channel, eight-channel micropipette and tip (10μL,.200μL).
5.4 Nitric acid microporous membrane (0.22 μm).
5.5 Micro Cell Culture Plate (96 well).
5.6 Steam sterilizer.
5.7 Surgical scissors, tweezers, pencils (HB), rulers.
6 reagents
The reagents used in this section are analytically pure reagents unless otherwise specified.
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