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SN/T 1680-2005: Protocol of diagnosis for acariosis of bees
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Protocol of diagnosis for acariosis of bees
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Basic data
| Standard ID | SN/T 1680-2005 (SN/T1680-2005) |
| Description (Translated English) | Protocol of diagnosis for acariosis of bees |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | B47 |
| Classification of International Standard | 65.140; 65.020.30 |
| Word Count Estimation | 6,672 |
| Date of Issue | 2005-09-30 |
| Date of Implementation | 2006-05-01 |
| Regulation (derived from) | Industry standard filing Notice 2005 No. 12 (No. 72 overall) |
| Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China |
| Summary | This standard specifies the shield bee bee mite Vu Thi diagnosis method. This standard applies to Vu Thi bee bee mite disease quarantine shield. |
SN/T 1680-2005: Protocol of diagnosis for acariosis of bees
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of diagnosis for acariosis of bees
People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard
Method for diagnosis of honeybee bee shield rickets
Released on September 15,.2005
2006-05-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard refers to the.2004 edition of the OIE Manual for Diagnostic Reagents and Vaccines for Terrestrial Animals.
Appendix A of this standard is a normative appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Beijing Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Shi Xia Ling, Qiao Jun, Su Shimin.
This standard is the first industry standard for entry-exit inspection and quarantine.
Method for diagnosis of honeybee bee shield rickets
1 Scope
This standard specifies the diagnostic method for the bee sting Shield.
This standard applies to the quarantine of the bee sting Shield.
2 Principle
The beeosis of bees is caused by the bee mites. Is an in vivo transmission of the bee's respiratory system
The worms live and reproduce in the chest and trachea of the bees, feed on the host's hemolymph, and sometimes parasitize the head, chest and abdomen. along with
As the number of parasitic mites increases, the trachea gradually develops lesions. Due to melanin, the original normal white translucent tracheal wall becomes non-
Transparent and with black spots, the trachea is filled with different stages of tracheal fistula.
3 equipment and reagents
The equipment and reagents that need to be prepared are.
---Optical microscope;
---Dissecting mirror;
---8% potassium hydroxide solution;
---1% methylene blue solution;
---70% alcohol.
4 methods and judgments
4.1 Anatomy
4.1.1 Randomly sample 50 bee samples (live, dead or sudden death), first kill with alcohol or freeze, place the bee body up, back down and supine
Or pinch with your thumb and forefinger.
4.1.2 Remove the head and forefoot with tweezers, remove the neck piece around the neck, open the exposed trachea, and see if the trachea closest to the stomata is infested.
4.1.3 Cut the chest between the middle leg and the front leg, and the thin disc is further processed to remove muscle tissue.
4.1.4 Set 8% potassium hydroxide solution (see Appendix A for solution preparation) and heat gently for about 20 minutes or without heating overnight.
4.1.5 Use a 18×~20× dissection mirror to examine the first pair of trachea with muscle tissue, or move the trachea to another slide with glycerin or water.
Observe at a higher magnification.
4.1.6 Small oval mites can be easily seen through the transparent tracheal wall.
4.1.7 Judgment. Under the anatomical microscope, it was found that the oval-shaped mites were positive in the trachea, and no oval mites were found in the trachea.
Is a negative result.
4.2 Staining method
4.2.1 Remove the head and front legs.
4.2.2 A pair of legs in the middle of the base of the front wing are then cut transversely to the front.
4.2.3 Remove the slice from 1mm to 1.5mm and set 8% potassium hydroxide solution.
4.2.4 Gently stir and heat until near the boiling point for about 10 minutes until the internal tissue is dissolved and removed. Do not move the chitin tissue.
4.2.5 Filter and collect the sections and rinse with tap water for 2 min to 5 min.
4.2.6 Staining. Dyeing with 1% methylene blue (see Appendix A for solution preparation), sectioning in distilled water for 2 min to 5 min, with 70% wine
Fine rinse, when stored in 95% alcohol, can be further colored for 6h.
