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SN/T 1618-2017: Detection and identification of Prunus necrotic ringspot virus
Status: Valid SN/T 1618: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1618-2017 | English | 259 |
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Detection and identification of Prunus necrotic ringspot virus
| Valid |
SN/T 1618-2017
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| SN/T 1618-2005 | English | 399 |
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Detection of prunus necrotic ringspot virus
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SN/T 1618-2005
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PDF similar to SN/T 1618-2017
Basic data | Standard ID | SN/T 1618-2017 (SN/T1618-2017) | | Description (Translated English) | Detection and identification of Prunus necrotic ringspot virus | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020 | | Word Count Estimation | 11,113 | | Date of Issue | 2017-07-21 | | Date of Implementation | 2018-03-01 | | Older Standard (superseded by this standard) | SN/T 1618-2005 | | Regulation (derived from) | State-Quality-Inspection-Accreditation (2017) 337 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the quarantine and identification methods for Prunus necrotic ring spot virus in plant quarantine. This standard applies to the quarantine and identification of Prunus necrotic ring spot virus in plant materials. | SN/T 1618-2005: Detection of prunus necrotic ringspot virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection of prunus necrotic ringspot virus
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
Prunus necrotic ring spot virus detection method
Released on.2005-08-18
2006-02-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
Appendix A of this standard is a normative appendix, and Appendix B and Appendix C are informative appendices.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted by the Animal and Plant Quarantine Research Institute of China Academy of Inspection and Quarantine, Beijing Entry and Exit Inspection of the People's Republic of China
The Quarantine Bureau and the Shaanxi Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China participated in the drafting.
The main drafters of this standard. Li Guifen, Li Mingfu, Wei Meisheng, Xiang Ning, Zhang Yongjiang, Han Lijuan, Wei Digong.
This standard is the first industry standard for entry-exit inspection and quarantine.
Prunus necrotic ring spot virus detection method
1 Scope
This standard specifies the basic principles and methods for the detection and identification of Prunus necrotic ring spot virus.
This standard applies to the detection of vegetative propagation materials, tissue culture seedlings, seeds and fruits of all incoming environments that may carry the Prunus necrotic ring spot virus.
And identification.
2 Principle
2.1 Lithium necrotic ring spot virus scientific name, taxonomic status
Scientific name. Prunusnecroticringspotvirus
Abbreviation. PNRSV
2.2 Host range
The virus has a wide range of hosts, and natural and experimental inoculation infects 21 families of dicots, which can infect most of the genus Prunus.
Rose, hops. In the case of artificial inoculation, more than 180 plants can be infested.
2.3 Disease symptoms
Symptoms of the disease vary depending on the isolate, cultivar and environmental conditions. Some isolates of the virus do not cause symptoms, only in the vaccination
Infection can only be detected when plants are detected by serological methods; some isolates produce necrotic spots on young leaves in the year of system infection.
And pores, but in later years, there are few symptoms on the leaves and fruits; other isolates produce necrotic reactions in the year of infection, with
It is followed by chronic chlorotic mottled and necrotic, leafy ear processes, malformations, delayed fruit ripening, and spotted symptoms on the fruit. As appearing on sweet cherries
From asymptomatic to severe wrinkled mosaic symptoms, chronic symptoms include chlorotic spots on the leaves, leaf twists, and delayed fruit ripening for days to weeks.
The shape varies depending on the virus isolate. Asymptomatic infection on roses is common, with symptoms such as mosaic, delayed flowering, early autumn leaves, and more
Many unformed flowers, infected plants are usually inactive. A chlorotic line and a ring spot are produced on the hop.
2.4 Distribution area
Widely distributed in temperate regions, such as in Europe and the United States.
2.5 Mode of transmission
Mechanical inoculation, pollen transmission, and seed transmission can also be carried out by artificial means such as asexual propagation of seedlings and transportation of tissue culture seedlings.
spread.
2.6 granule morphology
The genus Necrotic ring spot virus granules are equiaxed spheroids with diameters ranging from 23 nm to 27 nm, and some granules are quasi-equal spherical to short rod-shaped.
(Axis ratio is 1.01 to 1.5); virions of some strains are obviously rod-shaped (axial ratio greater than 2.2); some rod-shaped granules reach 70 nm. Baton
The presence or absence of granules and the proportion vary depending on the strain.
2.7 Viral genome
Positive single-stranded RNA, 3-part genome, RNA-1 is 3.662 kb long, RNA-2 is 2.507 kb long, and RNA-3 is 1.887 kb long.
3 Instruments, equipment and reagents
3.1 Equipment
Enzyme label detector, balance (1/10000g), PCR instrument, electrophoresis instrument, electrophoresis tank, UV transilluminator, isolation greenhouse, juicer, water bath
Pot and so on.
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