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SN/T 1616-2013 English PDF

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SN/T 1616-2013: Detection of African cassava mosaic virus
Status: Valid

SN/T 1616: Evolution and historical versions

Standard IDContents [version]USDSTEP2[PDF] delivered inStandard Title (Description)StatusPDF
SN/T 1616-2013English389 Add to Cart 3 days [Need to translate] Detection of African cassava mosaic virus Valid SN/T 1616-2013
SN/T 1616-2005English359 Add to Cart 3 days [Need to translate] African cassava mosaic virus detection method Obsolete SN/T 1616-2005

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Basic data

Standard ID SN/T 1616-2013 (SN/T1616-2013)
Description (Translated English) Detection of African cassava mosaic virus
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B16
Classification of International Standard 65.020
Word Count Estimation 15,114
Older Standard (superseded by this standard) SN/T 1616-2005
Quoted Standard SN/T 1840
Regulation (derived from) National quality recognition (2013), No. 464; industry standard filing Notice No. 1 of 2014 (No. 169 overall)
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the basic principles and methods of African cassava mosaic virus detection side identification.

SN/T 1616-2013: Detection of African cassava mosaic virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection of African cassava mosaic virus People's Republic of China Entry-Exit Inspection and Quarantine Standards Instead of the SN/T 1616-2005 African cassava mosaic virus detection methods Issued on. 2013-08-30 2014-03-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. Instead of the standard SN/T 1616-2005 "African cassava mosaic virus detection methods." This standard compared with SN/T 1616-2005, in addition to editorial changes outside the main technical changes are as follows. --- Increasing the conventional PCR detection method; --- Increasing the real-time PCR detection method. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. China Inspection and Quarantine Science Research Institute, People's Republic of China Guangdong CIQ. The main drafters of this standard. Li Mingfu, Feng Lixia, Lu Jie, Zhang Yongjiang, Jun hole, MATURAL, Xiangning, Weimei Sheng, Zhang ChengLiang. This standard replaces the standards previously issued as follows. --- SN/T 1616-2005. African cassava mosaic virus detection methods

1 Scope

This standard specifies the basic principles and methods of African cassava mosaic virus detection and identification of. This standard applies to all entry and cassava seedlings may carry the African cassava mosaic virus like quarantine detection and identification.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. SN/T 1840 plant virus immune electron microscopy method

3 African cassava mosaic virus Basic Information

Name. Africancassavamosaicvirus. Abbreviation. ACMV. Taxonomic position. The twin virus family (Geminiviridae) Bean golden mosaic virus genus (Begomovirus). Transmission. mainly through cassava whitefly (Bemisiatabaci), potato tubers and other communication. For additional information, see Appendix A. African cassava mosaic virus

4 principle of the method

Perform biological assays based on ACMV symptoms on differential hosts; and according to specific reactions between antibodies and ACMV of sample Products enzyme-linked immunosorbent assay; immunization mitochondrial electron microscope according to the characteristics of ACMV; special ACMV nucleic acid according to the conserved regions Heterosexual conventional PCR or real-time PCR assay, the result is determined by the size or electrophoretic bands of the amplification curve. 5 equipment, appliances, and reagents 5.1 Equipment Transmission electron microscopy, ELISA detector, electronic balance, water bath, PCR amplification, real-time PCR instrument, electrophoresis, desktop High-speed refrigerated centrifuge and gel imaging systems. 5.2 Appliances Adjustable pipette pipette head, ELISA plates, tubes and mortar and the like. 5.3 Reagents Unless otherwise stated, all test reagents were analytical grade or biochemical reagents. Detection reagents in Appendix B, Appendix C, Appendix D.

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