US$349.00 · In stock Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. SN/T 1617-2017: Detection and identification of Cacao swollen shoot virus Status: Valid SN/T 1617: Evolution and historical versions
Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
SN/T 1617-2017 | English | 349 |
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Detection and identification of Cacao swollen shoot virus
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SN/T 1617-2017
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SN/T 1617-2005 | English | 439 |
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Detection of Cacao swollen shoot virus
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SN/T 1617-2005
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PDF similar to SN/T 1617-2017
Basic data Standard ID | SN/T 1617-2017 (SN/T1617-2017) | Description (Translated English) | Detection and identification of Cacao swollen shoot virus | Sector / Industry | Commodity Inspection Standard (Recommended) | Classification of Chinese Standard | B16 | Classification of International Standard | 65.020 | Word Count Estimation | 15,155 | Date of Issue | 2017-07-21 | Date of Implementation | 2018-03-01 | Older Standard (superseded by this standard) | SN/T 1617-2005 | Regulation (derived from) | State-Quality-Inspection-Accreditation (2017) 337 | Issuing agency(ies) | General Administration of Customs | Summary | This standard specifies the detection method of cocoa swollen virus. This standard applies to the quarantine test of cocoa swollen virus in imported plant materials. |
SN/T 1617-2017: Detection and identification of Cacao swollen shoot virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Cacao swollen shoot virus
People's Republic of China entry and exit inspection and quarantine industry standards
Replace SN/T 1617-2005
Cocoa swollen virus quarantine and identification method
Released on.2017-07-21
2018-03-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard replaces SN/T 1617-2005 "Detection method of cocoa swollen virus", compared with SN/T 1617-2005, the main technical changes
The following is as follows.
--- Improve the original standard detection method and increase the real-time fluorescent PCR detection method;
--- Improved the original standard genomic content;
--- Removed the immuno-trap PCR method from the original standard and added the PCR method;
--- Revised the judgment of the results in the original standard;
--- Modified the appendix of the original standard.
Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. China Academy of Inspection and Quarantine, Zhongshan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, the Chinese People
And the Jiangsu Entry-Exit Inspection and Quarantine Bureau, Tianjin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Wei Meisheng, Chen Dinghu, Xiang Ning, Li Guifen, Wu Cuiping, Guo Jingze.
The previous versions of the standards replaced by this standard are.
---SN/T 1617-2005.
Cocoa swollen virus quarantine and identification method
1 Scope
This standard specifies the detection method of cocoa swollen virus.
This standard applies to the quarantine test of cocoa swollen virus in imported plant materials.
2 basic information of cocoa swollen virus
Scientific name. Cacaoswollenshootvirus
Abbreviation. CSSV
Chinese name. Cocoa swollen virus
Synonym. Theobromavirus1; Marmortheobromae; Theobromavirusinflans.
Classification status. Caulimoviridae; Badnavirus.
See Appendix A for additional information on CSSV.
3 Principle of the method
The protein and genomic characteristics of CSSV are the basis for the quarantine and identification of this virus. According to the specific reaction between CSSV and antibody,
Plant samples were subjected to double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA); PCR was performed based on the genomic characteristics of CSSV, real-time
Fluorescent PCR; by an effective combination of these methods, determine if the sample carries CSSV.
4 instruments, appliances and reagents
4.1 Instrumentation
Enzyme-linked detector, balance (sensitivity 0.001g), PCR instrument, electrophoresis instrument, real-time fluorescence PCR instrument, electrophoresis tank, UV transilluminator, isolation temperature
Room, juicer, water bath, etc.
4.2 Appliances
Adjustable pipettes (200μL, 20μL, 2μL), adjustable pipette tips, enzyme plates, centrifuge tubes, finger tubes, mortars, etc.
4.3 Reagents
Enzyme-linked assay reagents (see Appendix B) and polymerase chain reaction assay reagents (see Appendix C).
5 Detection methods
5.1 Double antibody sandwich enzyme-linked immunosorbent assay
See Appendix B.
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