SN/T 1612: Evolution and historical versions
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| SN/T 1612-2013 | English | 639 |
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Detection and identification of Carnation ringspot virus
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SN/T 1612-2013
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| SN/T 1612-2005 | English | 239 |
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Carnation ringspot virus serological detection methods
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SN/T 1612-2005
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PDF similar to SN/T 1612-2013
Basic data | Standard ID | SN/T 1612-2013 (SN/T1612-2013) | | Description (Translated English) | Detection and identification of Carnation ringspot virus | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020 | | Word Count Estimation | 16,124 | | Older Standard (superseded by this standard) | SN/T 1612-2005 | | Quoted Standard | SN/T 1840 | | Regulation (derived from) | National quality recognition (2013) 569 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the method of entry quarantine identified seedlings in Carnation ringspot virus DAS-ELISA, immuno-electron microscopy, immuno-capture and real-time RT-PCR and RT-PCR. This standard applies to the quarantine identify seedlings in Ca | SN/T 1612-2013: Detection and identification of Carnation ringspot virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Carnation ringspot virus
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Instead of the SN/T 1612-2005
Carnation ringspot virus quarantine and identification methods
Issued on. 2013-11-06
2014-06-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Instead of the standard SN/T 1612-2005 "Carnation ringspot virus serological detection methods."
This standard compared with SN/T 1612-2005, the main technical changes are as follows.
--- Increased immuno-capture by RT-PCR method;
--- Increased immune electron microscopy method;
--- Adds real-time fluorescent RT-PCR detection method;
--- Added Appendix Carnation ringspot virus related information.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard by the People's Republic of China Shanghai Exit Inspection and Quarantine is responsible for drafting.
The main drafters of this standard. Yang Cuiyun at Chui, Hupei Long, Tao Tingdian, Cuixue Hui, in the sub-Xiang.
This standard replaces the standards previously issued as follows.
--- SN/T 1612-2005.
Carnation ringspot virus quarantine and identification methods
1 Scope
This standard specifies the entry seedlings in Carnation ringspot virus DAS-ELISA, immuno-electron microscopy, immuno-capture RT-PCR and real
Quarantine and identification of fluorescent RT-PCR and the like.
This standard applies to the quarantine seedlings in the identification of Carnation ringspot virus.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
SN/T 1840 plant virus immune electron microscopy method
3 Carnation ringspot virus basic information
Chinese name. Carnation ringspot virus
English name. Carnationringspotvirus
Abbreviation. CRSV
Taxonomic position. tomato bushy stunt virus family (Tombusviridae), carnation ringspot virus genus (Dianthovirus)
Transmission. The virus spread through long distance transport of vegetative propagation material, mainly due to the close contacts between plants, soil
And soil pollution caused by farming operations spread of the virus.
For additional information, see Appendix Carnation ringspot virus A.
4 principle of the method
Carnation ringspot virus has a moderate to strong immunogenicity, easy to obtain high-quality multi antiserum weaker between serum within the genus virus
Cross-reactive.
In Carnation ringspot virus antiserum, using DAS-ELISA or immuno-capture RT-PCR screening for viruses, if the sample is positive
Resistance, the real-time fluorescent RT-PCR, immune electron microscopy, or differential host reactions, positive material verification. And two kinds of the above methods
Identification of the type of virus quarantine.
5 equipment, appliances and reagents
5.1 Equipment
Microplate reader, PCR, real-time PCR instrument, clean benches, electronic scales (1/10000g), electrophoresis, electrophoresis tank, gel imaging
System, water bath, high-speed refrigerated centrifuge, -80 ℃ ultra-low temperature refrigerators, autoclaves, ice maker, microwave, Vortex.
5.2 Appliances
Adjustable micropipette (2μL, 10μL, 100μL, 200μL, 1000μL, 5000μL) and the corresponding RNase-free tip, no
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