SN/T 1573: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1573-2013 | English | 359 |
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Determination of neurotoxic shellfish poisons in shellfish for export. Mouse biology method
| Obsolete |
SN/T 1573-2013
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| SN/T 1573-2005 | English | 679 |
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Neurological shellfish toxins in shellfish testing methods mouse bioassay
| Obsolete |
SN/T 1573-2005
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PDF similar to SN/T 1573-2013
Basic data | Standard ID | SN/T 1573-2013 (SN/T1573-2013) | | Description (Translated English) | Determination of neurotoxic shellfish poisons in shellfish for export. Mouse biology method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B51 | | Classification of International Standard | 67.120.30 | | Word Count Estimation | 9,951 | | Older Standard (superseded by this standard) | SN/T 1573-2005 | | Quoted Standard | GB/T 6682; GB 14925 | | Regulation (derived from) | National quality recognition (2013) 569 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the method for the determination of mouse neural biological shellfish and their products shellfish toxins. This standard applies to shellfish and shellfish products nerve toxin detection. | SN/T 1573-2013: Determination of neurotoxic shellfish poisons in shellfish for export. Mouse biology method
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Determination of neurotoxic shellfish poisons in shellfish for export.Mouse biology method
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Instead of the SN/T 1573-2005
Export shellfish toxins in shellfish nerve detection method
Mouse bioassay
Issued on. 2013-11-06
2014-06-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Release mechanism of the present document does not assume responsibility for the identification of these patents.
Instead of the standard SN/T 1573-2005 "shellfish toxins in shellfish nervous mouse bioassay test method."
This standard compared with SN/T 1573-2005, in addition to editorial changes, the major technical changes are as follows.
--- Increase normative references, reference standard APHA, 1985;
--- Modify the terms and definitions are defined in part on mouse units;
--- Modify part sample storage and preparation;
--- Modify the part measurement method;
--- Modify the part of the results expressed;
--- Increasing the health and safety content.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Liaoning Province Exit Inspection and Quarantine.
The main drafters of this standard. Zhao Xin, Wangqiu Yan, Xu Yang, Cheng Chiu, Qu Fei, Xu Jing, Cao Juan occasion.
This standard replaces the standards previously issued as follows.
--- SN/T 1573-2005.
Export shellfish toxins in shellfish nerve detection method
Mouse bioassay
1 Scope
This standard specifies the method for determination of mouse bioassay shellfish and their products in neuropathic shellfish toxins.
This standard applies to shellfish and shellfish products nerve toxin detection.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB 14925 Laboratory Animal Facilities and Environment
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Neural shellfish toxins Neurotoxicshelfishpoisons; NSP
The chemical structure of short naked dinoflagellate toxin (brevetoxin) represented after ingestion can produce neurological and gastrointestinal symptoms in the presence of shellfish meat
Marine life toxic substances in general.
3.2
Rat units mouseunit; MU
After weighing 20g mice by intraperitoneal injection of 1mL shellfish extract within 930min minimum required amount of toxins death
A mouse units
3.3
Median mediandata
The value of the variable according to the size of the order of, in the middle position is that the value of the median.
4 Method summary
NSP extracted with ether shellfish toxins, evaporated to dryness under reduced pressure, then 1% Tween -60 saline as a dispersion medium to prepare NSP-
1% Tween -60 saline suspension, the suspension was injected into the abdominal cavity, the mice were observed for survival, calculate its virulence.
5 Reagents and materials
Unless otherwise specified, reagents were analytical grade or biochemical reagents. Test water should comply with GB/T 6682 in a water specifications.
5.1 Concentrated hydrochloric acid
5.2 anhydrous ether.
5.31% Tween -60 NS. Weigh 1.0g Tween -60, dissolved in physiological saline (0.85% NaCl), and the volume to 100.0mL.
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