SN/T 1150: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1150-2015 | English | 559 |
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Detection and identification of Arabis mosaic virus
| Obsolete |
SN/T 1150-2015
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| SN/T 1150-2002 | English | 399 |
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Plant quarantine. Methods for inspection and identification of Arabis mosaic Nepovirus
| Obsolete |
SN/T 1150-2002
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PDF similar to SN/T 1150-2015
Basic data | Standard ID | SN/T 1150-2015 (SN/T1150-2015) | | Description (Translated English) | Detection and identification of Arabis mosaic virus | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Word Count Estimation | 14,185 | | Date of Issue | 2015-12-04 | | Date of Implementation | 2016-07-01 | | Older Standard (superseded by this standard) | SN/T 1150-2002 | | Regulation (derived from) | AQSIQ Announcement 2015 No.4th batch 120 items of Industry Standards | | Issuing agency(ies) | General Administration of Customs | SN/T 1150-2002: Plant quarantine. Methods for inspection and identification of Arabis mosaic Nepovirus
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Plant quarantine.Methods for inspection and identification of Arabis mosaic Nepovirus
People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard
Phytosanitary
Southern mustard mosaic virus quarantine and identification method
Released on November 11,.2002
2003-05-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard determines the blood according to the morphological, biological and serological characteristics of the Arabidopsis mosaic virus, combined with the characteristics of plant virus quarantine.
Qingxue DAS-ELISA and biological identification of the host as the main basis for quarantine identification of Arabidopsis mosaic virus. Participation in the development of this standard
It has compiled and compared many research results in recent years at home and abroad, and compiled it based on the experience of years of plant virus quarantine practice.
This standard is proposed and managed by the Certification and Accreditation Administration.
This standard was drafted. Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Wu Jiyun, Chen Zhinan, Deng Qiong, Wu Qiong.
This standard is the industry standard for inspection and quarantine issued for the first time.
Quarantine and identification method for phytosanitary mustard mosaic virus
1 Scope
This standard specifies the quarantine and identification methods for Arabidopsis mosaic virus in the entry phytosanitary.
This standard applies to the quarantine and identification of Arabidopsis mosaic virus in imported plant seeds, seedlings, propagation materials and plant products.
2 Principle
30nm, a member of the genus of the nematode polyhedrosis virus. Mainly distributed in most countries in Europe, Canada, Oceania and South Africa.
The virus has a wide range of hosts, about 174 genera and 215 species, including many cash crops such as melons, potatoes, tobacco, beans, cabbage, and flowers.
Broccoli, carrots, beets, cherries, peaches, grapes, strawberries, tulips, gladiolus and so on. According to the hazard symptoms, depending on the virus strain and host type
And the change, the virus particles are highly immunogenic, easy to prepare high titer virus antiserum, etc., serological double antibody sandwich method (DAS-
ELISA) and identification of host biological identification methods can be used as a basis for quarantine identification of the virus in quarantine work.
3 instruments
3.1 Research and Development
3.2 Micro Juicer
3.3 microplate reader
3.4 Low speed centrifuge
The speed is 4000r/min.
3.5 Biological incubator
With illumination, the temperature can be adjusted from 0 °C to 50 °C.
3.6 enzyme plate
48 or 96 wells.
3.7 Eppendorf tube
The volume is 1.5 mL.
3.8 Micropipette
Divided into 100μL,.200μL, 1000μL single head or 100μL or.200μL 8 channel and a corresponding tip.
3.9 constant temperature water bath or constant temperature incubator
3.10 Analytical electronic balance
3.11 pH meter
3.12 white porcelain plate (40cm × 50cm) and blunt tweezers
3.13 Isolation and quarantine temperature and net room
4 reagents
4.1 10XPBST buffer
80 g of sodium chloride (NaCl), 2 g of potassium dihydrogen phosphate (KH2PO4), 11.5 g of disodium hydrogen phosphate (Na2HPO4), potassium chloride (KCl)
2 g and Tween-20 (5 mL) were dissolved in 1000 mL of distilled water.
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