SN/T 1146: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1146-2010 | English | 359 |
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Detection and identification of tobacco ringspot virus
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SN/T 1146-2010
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| SN/T 1146-2002 | English | 359 |
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Plant quarantine. Methods for inspection and identification of tobacco ringspot Nepovirus
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SN/T 1146-2002
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PDF similar to SN/T 1146-2010
Basic data | Standard ID | SN/T 1146-2010 (SN/T1146-2010) | | Description (Translated English) | Detection and identification of tobacco ringspot virus | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.080 | | Word Count Estimation | 9,921 | | Date of Issue | 2010-05-27 | | Date of Implementation | 2010-12-01 | | Older Standard (superseded by this standard) | SN/T 1146-2002 | | Quoted Standard | SN/T 1840; SN/T 2122 | | Regulation (derived from) | National Quality Inspection (2010) 290; industry standard filing Notice 2010 No. 10 (No. 130 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the entry and exit of the tobacco plant quarantine in quarantine ringspot virus identification methods. This standard applies to entry and exit of plants and their products, tobacco ringspot virus quarantine identification. | SN/T 1146-2010: Detection and identification of tobacco ringspot virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of tobacco ringspot virus
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Instead of the SN/T 1146-2002
Tobacco ringspot virus quarantine and identification methods
Issued on. 2010-05-27
2010-12-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Instead of the standard SN/T 1146-2002 "tobacco ringspot virus quarantine and identification methods."
This standard compared with SN/T 1146-2002, the main technical changes are as follows.
--- Quarantine sampling site according to SN/T 2122-2008 "entry and exit quarantine of plants and plant products sampling" execution (see 6.2);
--- Remove the original standards Appendix A;
--- Added "symptom checker" one (see 6.3);
--- Immune electron microscopy method according to SN/T 1840-2006 "plant virus immune electron microscopy detection method" standard (see 7.3).
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China, Shenzhen CIQ.
The main drafters of this standard. Chen Chi-nan, Tang Qiong, Cheng Yun Ling apricot, Zheng Wenhua, Wang Dongyong.
This standard replaces the standards previously issued as follows.
--- SN/T 1146-2002.
Tobacco ringspot virus quarantine and identification methods
1 Scope
This standard specifies the export plant quarantine of tobacco ringspot virus in quarantine and identification methods.
This standard applies to the entry and exit of plants and their products in quarantine and identification of tobacco ringspot virus.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member, undated references, the latest edition (including any amendments) applies to this document.
SN/T 1840 plant virus immune electron microscopy method
SN/T 2122 entry and exit quarantine of plants and plant products sampling rules
Principle 3
Tobacco ringspot virus (tobaccoringspotvirus, referred TRSV), iso-called Anemone necrosis virus (anemonenecrosis
virus), blueberry necrotic ringspot virus (blueberrynecroticringspotvirus), tobacco ringspot virus No. 1 (tobaccoringspot
virus1). The virus is an isometric polyhedral spherical virus with a diameter of 25nm ~ 29nm, nematodes pass polyhedron virus is a member, can infect
54 Section 300 kinds of plants, the main cash crops are beans, potato, sweet potato, tobacco, watermelon, cucumbers, melons, carrots, Tangchang
Po, lily, iris, Prunus, apples, grapes, sweet cherries. Symptoms of plant infection by TRSV vary by host, usually appear blade ring
Or linear pattern, mottled or chlorotic spots, necrotic spots; root rot, stem dieback, are the fruit of malformations. The virus mainly rely on nematodes and seeds
Spread, mainly in North America, Oceania, Europe, Africa and other regions, with a broad host range, symptoms harm, immunogenicity, easy to make
Preparation of high titer, specifically the strong resistance of the virus antisera and other characteristics (see Appendix A). Serologically double antibody sandwich (DAS-ELISA) binding
Identification of host biological identification method or immune electron microscopy, as identified in the virus quarantine work basis.
4 utensils, equipment, facilities
Mortar, micro juicer, microplate reader, low-speed centrifuge (speed of 4000r/min), bio-incubator (with light, adjustable temperature range
Wai is 0 ℃ ~ 50 ℃), ELISA plate (48 or 96 wells), Eppendorf tubes (volume of 1.5mL), single channel pipette (10μL, 100
μL, 1000μL), 8-channel pipette (100μL or 200μL), heated water bath or incubator, analyze electronic balance, pH meter, white
Plate (40cm × 50cm), blunt forceps, quarantine temperature (net) chamber.
5 Reagents
5.1 10 × PBST buffer
Sodium chloride (NaCl) 80g, potassium dihydrogen phosphate (KH2PO4) 2g, disodium hydrogen phosphate (Na2HPO4) 11.5g, potassium chloride (KCl)
2g, Tween -20 (Tween-20) 5mL dissolved in distilled water, dilute to 1000mL.
5.2 Sample Extraction buffer
Sodium sulfite (Na2SO3) 1.3g, polyvinyl pyrrolidone (MW24000 ~ 40000, PVP) 20g, sodium azide (of NaN3)
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