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SN/T 1135.9-2010: Detection and identification of Ralstonia solanacearum (Smith) Yabuuchi et al.
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Detection and identification of Ralstonia solanacearum (Smith) Yabuuchi et al.
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SN/T 1135.9-2010
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Basic data | Standard ID | SN/T 1135.9-2010 (SN/T1135.9-2010) | | Description (Translated English) | Detection and identification of Ralstonia solanacearum (Smith) Yabuuchi et al. | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020 | | Word Count Estimation | 17,172 | | Date of Issue | 2010-11-01 | | Date of Implementation | 2011-05-01 | | Quoted Standard | GB/T 6682; SN/T 1157; SN/T 1193; SN/T 1809; SN/T 2122 | | Regulation (derived from) | National Quality Inspection (2010) 582 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the entry and exit phytosanitary bacterial wilt in potato Ralstonia solanacearum race 3 biovar 2 detection and identification methods. This standard applies to the entry and exit of potato tubers and its products, and its products and geranium tomato seedlings and seed potato products for the detection and identification of R. solanacearum. | SN/T 1135.9-2010: Detection and identification of Ralstonia solanacearum (Smith) Yabuuchi et al.---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Ralstonia solanacearum (Smith) Yabuuchi et al.
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Quarantine and identification of potato bacterial wilt
Issued on. 2010-11-01
2011-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 1135 standard is divided into the following sections.
--- Potato quarantine and identification of cancer disease;
--- Potato yellow dwarf virus quarantine and identification methods;
--- Potato mop top virus quarantine and identification methods;
--- Potato smut quarantine and identification of bacteria;
--- Potato Ring Rot quarantine and identification methods;
--- Potato Fei Rot quarantine and identification methods;
--- Potato A virus quarantine and identification methods;
--- Potato gangrene bacteria quarantine and identification methods;
--- Potato bacterial wilt quarantine and identification methods.
This section SN/T 1135 Part 9.
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section was drafted by. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine of Liaoning People's Republic of China
Exit Inspection and Quarantine and the People's Republic of China Heilongjiang.
The main drafters of this section. Yi Jianping, Zhou Guoliang, India Liping, Wang Youfu at constant pure.
Quarantine and identification of potato bacterial wilt
1 Scope
SN/T 1135 provisions of this part of the export plant quarantine potato bacterial wilt Ralstoniasolanacearumrace3
biovar2 detection and identification methods.
This section applies to the entry and exit of potato tubers and their products, and its products and tomato seedling geranium seed potato and green products
Dry detection and identification of bacteria.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member, undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
SN/T 1157 export plant seedlings quarantine rules
SN/T 1193 genetic testing laboratory technical requirements
SN/T 1809 export plant quarantine rules for seed
SN/T 2122 entry and exit quarantine of plants and plant products sampled
Principle 3
R.solanacearumrace3biovar2 potato bacterial wilt bacterium domain Bacteria, Proteobacteria Proteobacteria, β change
Shaped bacteria Gang Betaproteobacteria, Burkholderia bacteria head Burkholderiales, Burkholderia bacteria Branch Burkholderiaceae, Raul Escherichia
Ralstonia. Distribution, host range, damage symptoms and physiological and biochemical characteristics of potato bacterial wilt and other related information, see Appendix A. root
According solanacearum pathogenicity differences on different host plants of Ralstonia bacteria divided into five small species (see Appendix B), potato bacterial wilt bacterium belongs
3 small species, mainly infect potato and tomato. According to the three bacterial wilt disaccharide (maltose, lactose and cellobiose) and three kinds of ethanol
(Mannitol, sorbitol and dulcitol) difference oxidizing acid production capacity will be divided into four R. solanacearum biotypes (see Appendix B), potato bacterial wilt
Bacteria belonging to biotype 2 (only three kinds of oxide disaccharide, not three kinds of ethanol oxidation). Morphological characteristics of potato bacterial wilt, biological characteristics,
Pathogenic reactions and molecular biological characteristics as quarantine and identification of the enactment of this standard basis.
4 equipment and appliances
Clean table, autoclaves, bio-incubator, electronic scales, refrigerators, centrifuges, water bath, petri dishes, flasks, scissors, PCR
Instrument, real-time PCR instrument, electrophoresis, gel imaging system, BIOLOG microbial identification systems, adjustable micro pipettes and centrifuge tubes.
5 Drug agents
Unless otherwise specified, all reagents were of analytical grade or biochemical reagents.
Peptone, glycerol, magnesium sulfate, dipotassium phosphate, agar, TaqDNA polymerase, ethidium bromide (the EB), and DNA agarose
Marker. SMSA media formulations Appendix C, ELISA test reagents necessary drugs in Appendix D, PCR and real-time PCR test
Drug test reagents required in Appendix E.
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