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SN/T 1135.7-2009: Quarantine identification of Potato virus A
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| SN/T 1135.7-2009 | English | 259 |
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Quarantine identification of Potato virus A
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SN/T 1135.7-2009
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Basic data | Standard ID | SN/T 1135.7-2009 (SN/T1135.7-2009) | | Description (Translated English) | Quarantine identification of Potato virus A | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020 | | Word Count Estimation | 10,189 | | Date of Issue | 2009-09-02 | | Date of Implementation | 2010-03-16 | | Quoted Standard | SN/T 2122 | | Regulation (derived from) | Entry-Exit Inspection and Quarantine circular industry standards (2009. 09. 02) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the entry plant quarantine in the potato virus A quarantine identification. This standard applies to all entry seed, commodities with potato tissue culture and virus-free seedlings and other potato germplasm identification of potato virus A quarantine. | SN/T 1135.7-2009: Quarantine identification of Potato virus A---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine identification of Potato virus A
Exit inspection and quarantine industry standard book People's Republic of China
A quarantine and identification of potato virus
Posted 2009-09-02
2010-03-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
The Standard Appendix B, Appendix C, Appendix D is a normative appendix, Appendix A is an information appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard by the People's Republic of China Liaoning Entry Inspection and Quarantine Bureau, Chinese Academy of Inspection and Quarantine is responsible for drafting.
The main drafters of this standard. Wang Xiufen, Li Mingfu, Liu Wei, Gao Fang, Liushan Bin, Zhou Ming.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
A quarantine and identification of potato virus
1 Scope
This standard specifies the entry in the phytosanitary quarantine and identification of potato A virus.
This standard applies to all entry potato, with potato commodity, tissue culture and virus-free seedlings and other potato germplasm in potato A virus quarantine
Identification.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
SN/T 2122 entry and exit quarantine of plants and plant products sampled
Principle 3
3.1 scientific name and taxonomic status
Name. PotatovirusA
Acronym. PVA
3.2 based quarantine and identification
Serological Characteristics of Potato virus A, features, and biological characteristics of molecular biology is the main basis for quarantine and identification of.
Additional information see Appendix A.
4 equipment, appliances, and reagents
4.1 Equipment
Enzyme-linked detector, the balance (a sense of volume, 1/10000g), pH meter, trace juicer, PCR instrument, electrophoresis, electrophoresis tank, gel imaging system,
Greenhouse isolation, constant temperature water bath, cold refrigerator.
Micropipette (2μL, 10μL, 20μL, 100μL, 200μL, 1000μL), ELISA plate, mortar, eppendorf tubes, flower pots, disinfection
Soil.
4.2 Reagents
Enzyme-linked immunosorbent assay reagent (see Appendix B), RT-PCR detection reagent (see Appendix C).
5 Sample Preparation
Sampling carried out in accordance SN/T 2122's.
Potato tubers grown in the greenhouse isolated, grown and symptoms observed at 25 ℃. Grow to be 3 to 4 leaves after disease manifestations
Plants like numbers do not show symptoms of the plant grouping (10 per group) and number. Leaves collected enzyme-linked immunosorbent assay, RT-PCR
Detection or biological assays.
6 Detection
6.1 ELISA
See Appendix B.
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