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Identification of Clavibacter michiganensis subsp.sepedonicus
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SN/T 1135.5-2007
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Basic data | Standard ID | SN/T 1135.5-2007 (SN/T1135.5-2007) | | Description (Translated English) | Identification of Clavibacter michiganensis subsp.sepedonicus | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020 | | Word Count Estimation | 9,920 | | Date of Issue | 2007-12-24 | | Date of Implementation | 2008-07-01 | | Regulation (derived from) | Industry standard filing Notice 2008 No. 2 (No. 98 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the potato ring rot pathogen quarantine and identification. This standard applies to all entry and exit of merchandise with potato and potato quarantine and identification. | SN/T 1135.5-2007: Identification of Clavibacter michiganensis subsp.sepedonicus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Identification of Clavibacter michiganensis subsp.sepedonicus
Exit inspection and quarantine industry standard book People's Republic of China
Potato Ring Rot quarantine and identification methods
Posted 2007-12-24
2008-07-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 1135 is divided into five parts.
--- Potato quarantine and identification of cancer disease;
--- Potato yellow dwarf virus quarantine and identification methods;
--- Potato mop top virus quarantine and identification methods;
--- Potato smut quarantine and identification of bacteria;
--- Potato Ring Rot quarantine and identification methods.
This section is SN/T 1135 Part 5.
This section Appendix B, Appendix C are normative appendices, Appendix A is an information appendix.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section drafted by. China Inspection and Quarantine Science Research Institute, Beijing People's Republic of China Exit Inspection and Quarantine.
The main drafters of this section. Zhao Wenjun, Chen Yun, Liang Miao, Zhang Yue, Zhu Shuifang, Weimei Sheng.
The first part of the Department of Inspection and Quarantine issued by industry standards.
Potato Ring Rot quarantine and identification methods
1 Scope
SN/T 1135 provisions of this part of the potato ring rot pathogen quarantine and identification methods.
This section applies to all entry and exit Seed Potato and merchandise using potato quarantine and identification of.
2 Terms and definitions
The following terms and definitions apply to SN/T 1135 to the present section.
2.1
Immunosorbent assay double antibody sandwich enzyme-linked
(ELISA plate) coated with bacteria-specific antibodies on the solid phase support, after the sample is added, and then an enzyme-labeled antibody for bacteria
Identification A serological detection of bacteria by detecting the presence or absence of the final enzymatic reactions.
2.2
PCR
In thermostable DNA polymerase and a pair of primers (tested with target nucleic acid molecule sequences homologous DNA fragments) at high temperature (DNA points
Sub-denatured) and low temperature (primers and the target nucleic acid molecule complex nature and thermostable DNA polymerase extension) alternating cycles of amplification of the target nucleic acid test
Child approach.
Principle 3
4 apparatus and appliances
4.1 Equipment
PCR instrument, clean bench, sterilizer, ice maker, nucleic acid protein analyzer, high-speed refrigerated centrifuge, small desktop centrifuge, ultra-low temperature
Refrigerator, conventional refrigerator, Vortex, micro-injector, electrophoresis, gel imaging system, microplate reader.
4.2 Reagents
Unless otherwise specified, all reagents were of analytical grade. PCR buffer, magnesium chloride (MgCl2), dNTPs (dATP, dTTP,
dCTP, dGTP), T Rao q DNA polymerase, primers and probes, beef extract, yeast extract, dipotassium hydrogen phosphate (K2HPO4), potassium dihydrogen phosphate
(KH2PO4), magnesium sulfate (MgSO4), dextrose, agar, ELISA detection reagent in Appendix B.
Identify bacteria 5
5.1 Symptom Check
Take a sample amount of 1% to 5% (if less may be appropriate to increase the proportion of the sample) of tubers to be checked. The tubers cross check ring rot pathogen infection
Are symptoms, squeeze tubers, check the vascular tissue maceration. The incidence of vascular yellow light, was not continuous color dots; severe entire vascular bundle
Ring color. Bacteria can also be against tuber parenchyma around vascular bundle, a ring rot can cause severe skin and pith tissue isolation,
Gently squeeze cheese-like tubers gush of pillars from the vascular bundles. Symptoms tuber surface in mild hazard is not obvious, with the development of disease, skin
Or dark brown discoloration, severe skin cracks can occur.
5.2 pairs antibody sandwich enzyme-linked immune assay
The use of double-antibody sandwich enzyme-linked immunosorbent assay samples were screened (see Appendix B), if the test result is positive, the need for pathogens
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