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Detection and identification of potato virus V
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SN/T 1135.10-2013
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Basic data | Standard ID | SN/T 1135.10-2013 (SN/T1135.10-2013) | | Description (Translated English) | Detection and identification of potato virus V | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.99 | | Word Count Estimation | 16,149 | | Quoted Standard | SN/T 1840 | | Regulation (derived from) | National quality recognition (2013), No. 464; industry standard filing Notice No. 1 of 2014 (No. 169 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the basic principles and methods of potato virus quarantine V identification. This standard applies to all entry and exit quarantine and identification of seed, commodities with potatoes, potato tissue culture and virus-free seedli | SN/T 1135.10-2013: Detection and identification of potato virus V---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of potato virus V
ICS 65.020.99
B16
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Quarantine and identification of potato virus V
Issued on. 2013-08-30
2014-03-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 1135 is divided into 10 parts.
--- Part 1. Potato quarantine and identification of cancer disease;
--- Part 2. potato yellow dwarf virus quarantine and identification methods;
--- Part 3. Potato mop top virus quarantine and identification methods;
--- Part 4. potatoes smut quarantine and identification of bacteria;
--- Part 5. Potato Ring Rot quarantine and identification methods;
--- Part 6. Potato Rot Fei quarantine and identification methods;
--- Part 7. potato virus A quarantine and identification methods;
--- Part 8. potato gangrene quarantine and identification of bacteria;
--- Part 9. Potato quarantine and identification of bacterial wilt;
--- Part 10. V Potato Virus quarantine and identification methods.
This section SN/T 1135 Part 10.
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Jiangsu Entry-Exit Inspection and Quarantine Bureau, Chinese Academy of Inspection and Quarantine, the People's Republic of China
State and Ningbo Entry-Exit Inspection and Quarantine Yantai People's Republic of China, Heilongjiang People's Republic of China Entry-Exit Inspection
Inspection and Quarantine Bureau, People's Republic of China Liaoning Province Exit Inspection and Quarantine.
The main drafters of this standard. Li Bin, Wen Weigang, Su Zhi Ping, Tianyong Lei, Wu Cuiping, Su Han, Liu Hongyi, Wang Xiufen, Li Mingfu.
Quarantine and identification of potato virus V
1 Scope
This standard specifies the basic principles and methods of potato virus V quarantine identified.
This standard applies to all entry and exit potato, with potato commodity, tissue culture and virus-free seedlings and other potato and tomato germplasm and other Solanaceae crops may
V with quarantine and identification of potato virus.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
SN/T 1840 plant virus immune electron microscopy method
Principle 3
Potato virus V 3.1 Name and taxonomic status
Name. PotatovirusV
Abbreviation. PVV
Taxonomic position. potato virus Y Branch (Potyviridae) genus of potato virus Y (Potyvirus).
3.2 based quarantine and identification
Potato virus V serological, molecular and biological characteristics of the virus particle morphology is the main basis for quarantine and identification of.
The virus related information, see Appendix A.
4 equipment, appliances, and reagents
4.1 The main equipment
Enzyme-linked detector, washing machines, high-speed refrigerated centrifuge desktop, PCR, quantitative PCR instrument, electrophoresis, horizontal electrophoresis tank gel
Imager, water bath, transmission electron microscopy, the balance (the amount of sense. 0.001g) and the like.
4.2 major appliances
Mortar and pestle, a variety of range adjustable pipette (2μL, 10μL, 20μL, 100μL, 200μL, 1000μL), the various tip
(10μL, 200μL, 1000μL), 96 well microtiter plates, centrifuge tubes (0.2mL, 0.6mL, 1.5mL) and the like.
4.3 Reagents
The main enzyme-linked assay reagent see Appendix B, reverse transcription-polymerase chain reaction (RT-PCR) detection reagent Appendix C, real-time fluorescence RT-
PCR detection reagent in Appendix D, biological assay reagent see Appendix E.
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