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SN/T 0973-2010: Determination of entero-hemorrhagic E.coli O157: H7 in meats/meat product and other food for import and export
Status: Valid SN/T 0973: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 0973-2010 | English | 369 |
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Determination of entero-hemorrhagic E.coli O157: H7 in meats/meat product and other food for import and export
| Valid |
SN/T 0973-2010
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| SN/T 0973-2000 | English | 319 |
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Method for the determination of entero-hemorrhagic E. coli O157: H7 in meats and meat products for import and export and export
| Obsolete |
SN/T 0973-2000
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PDF similar to SN/T 0973-2010
Basic data | Standard ID | SN/T 0973-2010 (SN/T0973-2010) | | Description (Translated English) | Determination of entero-hemorrhagic E.coli O157: H7 in meats/meat product and other food for import and export | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X22 | | Word Count Estimation | 14,188 | | Date of Issue | 2010-11-01 | | Date of Implementation | 2011-05-01 | | Older Standard (superseded by this standard) | SN/T 0973-2000 | | Regulation (derived from) | National Quality Inspection (2010) 582 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the import and export of meat and meat products seedlings enterohemorrhagic Escherichia coli O157: H7 detection method. This standard applies to imports of meat, meat products and other foods EHEC O157: H7 qualitative test. | SN/T 0973-2010: Determination of entero-hemorrhagic E.coli O157: H7 in meats/meat product and other food for import and export
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of entero-hemorrhagic E.coli O157. H7 in meats/meat product and other food for import and export
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Instead of the SN/T 0973-2000
Import and export of meat, meat products and other food
The EHEC
O157. H7 Detection
E.coliO157. H7inmeats/meatproductandotherfoodforimportandexport
Issued on. 2010-11-01
2011-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Instead of the standard SN/T 0973-2000 "import and export meat and meat products EHEC O157. H7 detection method."
This standard compared with SN/T 0973-2000, the main technical changes are as follows.
--- Complements the ELISA method for rapid detection of EHEC O157. H7 in content;
--- Complements the molecular characteristics of microbial genetic identification techniques (genotyping) content, to facilitate the transfer of harmful pathogens traceability
Charles and variability of.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau, Shenzhen People's Republic of China Entry-Exit Inspection and Quarantine
Bureau, 3M China Ltd, bioMérieux (China) company.
The main drafters. Zhang Shuhong, Gu Ming, Han Wei, Lin Yang Jie, Fan release, Lu Jing Zhang, Wan Zhigang, Lvlei, Zhu Yi-hua, Liu Yi.
This standard replaces the standards previously issued as follows.
--- SN/T 0973-2000.
Import and export of meat, meat products and other food
The EHEC
O157. H7 Detection
1 Scope
This standard specifies the import and export of meat and meat products O157 EHEC. H7 Detection of.
This standard applies to the import and export of meat, meat products and other foods of EHEC O157. H7 is a qualitative test.
2 Definitions and Terminology
The following terms and definitions apply to this document.
2.1
EHEC O157. H7 entero-hemorrhagicE.ColiO157. H7
The bacteria are aerobic or facultative anaerobic, gram-negative, Zhou flagella, and pili, does not produce spores, fermentation of lactose, not fermented or slow
Sorbitol fermentation, oxidase negative, [beta] -glucosidase-negative bacilli.
3 PRINCIPLE OF THE METHOD
Tecra microplate method using Escherichia coli O157. H7 rapid detection kit 1). Sample enrichment broth boil cleaved lysates
After specific antibody (primary antibody) is a package microplate hybridization with enzyme-labeled antibody (secondary antibody) and then hybridized with a particular substrate and then reacted to form a colored compound
Thereof, or a microplate reader to detect with the naked eye.
4 Equipment and Materials
4.1 Incubator. (10 ℃ ~ 50 ℃) ± 1 ℃.
4.2 homogenizer. less than 12000r/min.
4.3 Autoclave.
4.4 CHROMagar E.coli O157. H7 chromogenic agar 2).
4.5 VIDAS automated enzyme immunoassay analyzer, France Meili Ai company's products or other equivalent products 3).
4.6 VITEK automatic biochemical microbial identification system or other equivalent product 3).
1), 2) respectively by the United States and France CHROMagar 3M company's trade name products. This information is given in order to facilitate the use of this standard
Who does not imply endorsement of the product. If other equivalent product has the same effect, you can use these equivalent products.
3) trade names provided by the French company bioMérieux products. This information is given for the convenience of users of this standard does not mean that the product
Recognition. If other equivalent product has the same effect, you can use these equivalent products.
4.7 General microbiology laboratory glassware, pipettes, glass dish and so on.
4.8 platinum-iridium or nickel-chromium wire inoculating loop diameter of about 3mm; L glass rod.
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