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SN/T 0670-2012 English PDF

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SN/T 0670-2012: Determination of tylosin residues in food for export. LC-MS/MS
Status: Valid

SN/T 0670: Evolution and historical versions

Standard IDContents [version]USDSTEP2[PDF] delivered inStandard Title (Description)StatusPDF
SN/T 0670-2012English519 Add to Cart 4 days [Need to translate] Determination of tylosin residues in food for export. LC-MS/MS Valid SN/T 0670-2012
SN 0670-1997English559 Add to Cart 4 days [Need to translate] Method for the determination of tylosin residues in eggs for export. Cylinder plate method Obsolete SN 0670-1997

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Basic data

Standard ID SN/T 0670-2012 (SN/T0670-2012)
Description (Translated English) Determination of tylosin residues in food for export. LC-MS/MS
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard X04
Classification of International Standard 67.050
Word Count Estimation 20,290
Older Standard (superseded by this standard) SN 0670-1997
Quoted Standard GB/T 6682
Regulation (derived from) National Quality Inspection (2012) 237
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the tylosin residues in food sample preparation and liquid chromatography mass spectrometry/mass spectrometry. This standard applies to shrimp, pork, liver, kidney, eggs, and milk tylosin residues detected.

SN/T 0670-2012: Determination of tylosin residues in food for export. LC-MS/MS

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of tylosin residues in food for export.LC-MS/MS People's Republic of China Entry-Exit Inspection and Quarantine Standards Replacing SN 0670-1997 Determination of tylosin residues in food exports Liquid chromatography - mass spectrometry/mass spectrometry LC-MS/MS Issued on. 2012-05-07 2012-11-16 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard replaces SN 0670-1997 "export eggs tylosin residues in plate method." This standard compared with SN 0670-1997, major technical changes as follows. --- Modify the standard name; --- Deletion of the sampling section. Please note that some of the content of this document may involve patents. Release mechanism of the present document does not assume responsibility for the identification of these patents. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Zhejiang Exit Inspection and Quarantine. The main drafters of this standard. Xie Wen, Ding Huiying, Qian Yan, Xijun Yang, Cai Jingyi. This standard replaces the standards previously issued as follows. --- SN 0670-1997. Determination of tylosin residues in food exports Liquid chromatography - mass spectrometry/mass spectrometry

1 Scope

This standard specifies the sample preparation and liquid chromatography tylosin residues in food - mass spectrometry/mass spectrometry. This standard applies to detect shrimp, pork, liver, kidney, eggs, and milk tylosin residues.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 analysis.

3 Method summary

Samples were extracted with methanol residual tylosin, C18 SPE column purification, liquid chromatography - mass spectrometry/mass spectrometry, external standard method Quantified.

4 Reagents and materials

Unless otherwise specified, all reagents were of analytical grade water as a water GB/T 6682 regulations. 4.1 acetonitrile. HPLC grade. 4.2 Methanol. HPLC grade. 4.3 formic acid. HPLC grade. 4.4 hexane. HPLC grade. 4.5 sodium hydroxide. 4.6 sodium dihydrogen phosphate. 4.7 0.1mol/L sodium hydroxide solution. Weigh 4g of sodium hydroxide, and diluted with water to 1L. 4.8 aqueous methanol solution. methanol - water (28, volume ratio). 4.9 Phosphate buffer solution. Weigh 13.8g of sodium dihydrogen phosphate, was dissolved in 950mL water and extracted with 0.1mol/L sodium hydroxide solution to adjust Solution to pH 8.0, and finally diluted with water to 1L. 4.10 tylosin tartrate standards (Tylosintartrate, CAS number. 74610-55-2, C50H83NO23). purity of greater than or equal to At 95%. 4.11 tylosin standard stock solution. learn the right amount of converted standard (4.10), dissolved in methanol solution at a concentration of 100μg/mL, 0 ℃ ~ 4 ℃ refrigerator stored. Valid for three months. 4.12 standard solution. A standard solution diluted according to need to use the blank sample solution to 10ng/mL, 12.5ng/mL, 25ng/mL, 75ng/mL, the standard working solution 125ng/mL, equivalent to a sample containing 20μg/kg, 25μg/kg, 50μg/kg, 150μg/kg, 250μg/kg tylosin. 4.13 C18 SPE column, 500mg, or rather who, prior to use successively with 5mL methanol, 5mL water and 5mL phosphate buffer solution

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