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LY/T 2896-2017: A regulation for giant panda population genetic archives
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A regulation for giant panda population genetic archives
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LY/T 2896-2017
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Basic data | Standard ID | LY/T 2896-2017 (LY/T2896-2017) | | Description (Translated English) | A regulation for giant panda population genetic archives | | Sector / Industry | Forestry Industry Standard (Recommended) | | Classification of Chinese Standard | B65 | | Classification of International Standard | 65.020 | | Word Count Estimation | 18,135 | | Date of Issue | 2017-10-27 | | Date of Implementation | 2018-01-01 | | Regulation (derived from) | State Forestry Administration Announces No. 18 of 2017; Industry Standards Record Announcement No. 12 of 2017 (Total No. 216) | | Issuing agency(ies) | State Forestry Administration | LY/T 2896-2017: A regulation for giant panda population genetic archives---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
A regulation for giant panda population genetic archives
ICS 65.020
B65
People's Republic of China Forestry Industry Standard
Technical regulations for the establishment of genetic records of giant panda population
Released on.2017-10-27
2018-01-01 implementation
State Forestry Administration released
Content
Foreword III
1 Scope 1
2 Terms and Definitions 1
3 sampling frequency, time and route 1
3.1 sampling frequency and time 1
3.2 Sampling route 1
4 Sample collection and preservation method 1
4.1 Sampling Tool 1
4.2 Judging the freshness of feces 2
4.3 Selection of stool samples 2
4.4 Sample Collection Method 2
4.5 Sample Record and Number 2
4.6 Temporary storage of samples 2
4.7 Sample transport and laboratory storage 3
5 Sample DNA extraction and preservation method 3
5.1 DNA extraction from stool samples 3
5.2 Preservation of DNA 3
6 Genetic marker selection principle 3
6.1 Mitochondrial markers 3
6.2 Sex Identification Molecular Mark 3
6.3 Microsatellite Marking 3
7 Experimental procedure 4
7.1 DNA quality detection and mitochondrial label PCR amplification 4
7.2 Individual Identification 4
7.3 Gender Identification 4
7.4 Genetic Diversity Assessment 4
8 Mitochondrial Labeling PCR Amplification Method 4
8.1 Primer Synthesis 4
8.2 PCR amplification system and procedures 4
9 Giant Panda Sex Identification PCR Method 5
9.1 Synthesis of PCR amplification primers 5
9.2 PCR amplification system and procedures 5
9.3 Electrophoresis detection 5
9.4 Gender Interpretation 5
10 microsatellite marker PCR amplification method 5
10.1 Design, Synthesis and Fluorescent Labeling of PCR Primers 5
10.2 PCR amplification reaction system and amplification procedure 5
10.3 Quality Inspection of PCR Products 6
10.4 Microsatellite Classification 6
10.5 Application of microsatellite typing data 6
11 Contents of the population genetic file 6
11.1 Sampled data 6
11.2 Mitochondrial marker sequencing data 6
11.3 Gender Identification Data 6
11.4 Microsatellite Marking Data 6
11.5 Research Report 6
12 Management and use of genetic archive data 7
Appendix A (Normative Appendix) Giant Panda Feces Sample Collection Record Table 8
Appendix B (Normative) Giant Panda Microsatellite Marking Number, Name, Core Sequence and Source 9
Appendix C (Normative) Giant Panda Microsatellite Tag Name, PCR Amplification Primer and Amplification Length 10
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the Protection Department of the State Forestry Administration.
This standard was drafted. Sichuan University, Institute of Zoology, Chinese Academy of Sciences, China Giant Panda Conservation Research Center, Chengdu Giant Panda Breeding Research
Base, Sichuan Wildlife Resources Survey and Protection Management Station.
The main drafters of this standard. Yue Bisong, Wei Fuwen, Li Desheng, Shen Fujun, Yang Xuyu, Gu Xiaodong, Zhang Ling, Chen Hao.
Technical regulations for the establishment of genetic records of giant panda population
1 Scope
This standard specifies the collection and preservation of giant panda samples, DNA extraction, gender identification, individual identification, genetic diversity assessment and population inheritance.
The techniques and methods of archival establishment.
This standard applies to captive giant pandas and wild giant pandas.
2 Terms and definitions
The following terms and definitions apply to this document.
2.1
Microsatellite sequence microsatelitesequence
A DNA tandem repeat consisting of one to six nucleotides of a basic repeating unit in the genome, such as (AC)n, (ACG)n,
(ATTT)n, etc., also known as short tandem repeats (STR) or simple repeats (simplesequences)
Repeats, SSR). A polymorphic microsatellite sequence that changes in the number of repetitions between individuals can be used as a microsatellite marker.
2.2
DNA sample DNAsamples
Giant panda genomic DNA extracted from giant panda tissue (such as hair, blood, semen, muscle tissue, etc.) or fresh feces.
3 sampling frequency, time and route
3.1 sampling frequency and time
The collection of wild giant panda samples can be carried out in conjunction with daily work such as protected area monitoring and patrolling.
Large-scale centralized sampling of wild giant panda samples can be carried out after spring snow (breeding season) and winter snow accumulation (non-breeding season);
In the densely distributed area of giant pandas with convenient transportation, the number of sampling times can be increased according to specific conditions and work targets. All localities can be based on local
Giant panda's habits, as well as local phenology, climate, bamboo shoots of giant panda staples, field working conditions and logistical support, etc.
Volume sampling time. Field sampling should be completed in the shortest possible time, usually 10d~20d.
Captive giant panda samples can be collected at any time in conjunction with daily work.
3.2 Sampling route
The large-scale centralized collection route of the sample is based on the fourth giant panda survey method in the country, and the habitat of the giant panda in the reserve is divided into
Several grids with an area of 2km2, each grid as a sampling cell, and numbered. In a single cell, according to the topography and the road
The actual sampling route is laid out by factors such as roads, rivers, vegetation, and giant pandas.
The daily collection of samples can be determined based on the monitoring patrol route.
4 Sample collection and preservation methods
4.1 Sampling tools
Handheld terminal equipment, satellite locator, topographic map, record form, disposable PE gloves, 500mL plastic sample bottle, analytical pure water
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