|
US$329.00 ยท In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
LY/T 2756-2016: Molecular identification method for fraxinus varieties with SRAP(sequence-related amplified polymorphism) molecular markers
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| LY/T 2756-2016 | English | 329 |
Add to Cart
|
3 days [Need to translate]
|
Molecular identification method for fraxinus varieties with SRAP(sequence-related amplified polymorphism) molecular markers
| Valid |
LY/T 2756-2016
|
PDF similar to LY/T 2756-2016
Basic data | Standard ID | LY/T 2756-2016 (LY/T2756-2016) | | Description (Translated English) | Molecular identification method for fraxinus varieties with SRAP(sequence-related amplified polymorphism) molecular markers | | Sector / Industry | Forestry Industry Standard (Recommended) | | Classification of Chinese Standard | B61 | | Classification of International Standard | 65.020 | | Word Count Estimation | 14,169 | | Date of Issue | 2016-10-19 | | Date of Implementation | 2017-01-01 | | Regulation (derived from) | State Forestry Administration Announcement No 2016 No.19 | | Issuing agency(ies) | State Forestry Administration | LY/T 2756-2016: Molecular identification method for fraxinus varieties with SRAP(sequence-related amplified polymorphism) molecular markers
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Molecular identification of waxy varieties)
ICS 65.020
B61
People's Republic of China Forestry Industry Standard
Molecular identification method for white wax varieties -
SRAP molecular marker
Released on October 18,.2016
2017-01-01 implementation
State Forestry Administration released
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard was proposed by the Forestry Department of Liaoning Province.
This standard is under the jurisdiction of the State Forestry Administration.
This standard was drafted. Liaoning Academy of Forestry, Shenyang Agricultural University, Jinzhou Forestry Research Institute.
The main drafters of this standard. Chen Yu, Fan Jungang, Wei Zhongping, Ye Jingfeng, Ma Dongjing, Guo Zhifu, Liu Ping, Liu Yifei, Meng Fanjin, Pan Wenli,
Yan Li, Feng Jian, Liu Hongmin, Wang Yuchun, Gao Yingxu, Yang He, Tian Yongxia, Wang Qin, Liu Wei, Yi Hongfeng, Jin Shan, Zhao Lihui.
Molecular identification method for white wax varieties -
SRAP molecular marker
1 Scope
This standard specifies the operating procedures and analytical methods for the molecular identification method of white wax varieties - SRAP molecular marker method.
This standard is applicable to the experimental process of identification of white wax varieties (species) by using the related sequence amplification polymorphism (SRAP) method.
2 Principle
The ash genomic DNA was amplified by designing a pair of primers. The forward primer (F-primer) contains 17 bases, exon
Specific amplification was carried out; the reverse primer (R-primer) contained 18 bases, and the intron region and the promoter region were amplified. Due to individual differences
And the introns of the species, the promoter and the length of the spacer are different to produce polymorphism. Ultimately by non-denaturing polyacrylamide gel electrophoresis
These specific polymorphic fragments were isolated and compared by comparing the differences in the DNA fingerprint bands of the test sample and the control variety.
Whether the test sample and the control variety are the same variety.
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Related sequence polymorphism-relatedamplifiedpolymorphism; SRAP
A pair of primers are used to amplify ORFs (openreading frames), a pair of primers including a forward primer and a counter
Polymorphisms are generated for primers that differ in their individual size and the length of the intron, promoter and spacer sequences of the species.
4 Instruments and equipment
4.1 PCR instrument.
4.2 Agarose gel electrophoresis system.
4.3 Non-denaturing polyacrylamide gel electrophoresis system.
4.4 Gel imaging system and camera system.
4.5 Ultrapure water system.
4.6 Low temperature high speed refrigerated centrifuge.
4.7 Ultra-micro UV/visible spectrophotometer.
4.8 Autoclave.
4.9 Liquid nitrogen tank.
4.10 Ultra-low temperature refrigerator.
4.11 High-voltage electrophoresis instrument (3000V voltage regulator) and supporting electrophoresis tank.
4.12 Pipette (gun).
Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of LY/T 2756-2016_English be delivered?Answer: Upon your order, we will start to translate LY/T 2756-2016_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time. Question 2: Can I share the purchased PDF of LY/T 2756-2016_English with my colleagues?Answer: Yes. The purchased PDF of LY/T 2756-2016_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to [email protected]. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.
|