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LST6146-2023: Inspection of grain and oils - Enumeration of molds in grain - Fluorescence rapid detection method
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| LS/T 6146-2023 | English | 199 |
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Inspection of grain and oils - Enumeration of molds in grain - Fluorescence rapid detection method
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LS/T 6146-2023
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Basic data | Standard ID | LS/T 6146-2023 (LS/T6146-2023) | | Description (Translated English) | Inspection of grain and oils - Enumeration of molds in grain - Fluorescence rapid detection method | | Sector / Industry | Food & Beverage Industry Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.060 | | Word Count Estimation | 10,129 | | Date of Issue | 2023-11-14 | | Date of Implementation | 2024-05-14 | | Issuing agency(ies) | National Grain and Material Reserves Administration | LST6146-2023: Inspection of grain and oils - Enumeration of molds in grain - Fluorescence rapid detection method
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 67.060
CCSX04
Grain Industry Standard of the People's Republic of China
Grain and Oil Inspection. Mold Count in Grains
Fluorescence rapid detection method
Released on 2023-11-14
2024-05-14 Implementation
The State Administration of Grain and Material Reserves released
Published by China Standards Press
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting.
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document was proposed by the State Administration of Grain and Material Reserves.
This document is under the jurisdiction of the National Technical Committee on Cereals and Oils Standardization (SAC/TC270).
This document was drafted by. National Grain and Material Reserves Administration Scientific Research Institute, National Grain and Material Reserves Administration Standard Quality Center, Shandong
Provincial Grain and Oil Testing Center, China National Grain Reserve Management Group Co., Ltd. Warehousing Management Department, Hebei Grain and Oil Quality Testing and Information Service Center, Tianjin
Tianjin Customs Animal and Plant and Food Inspection Center, Jilin Province Grain and Oil Hygiene Inspection and Monitoring Station, Guangdong Province Grain and Material Reserve Security Center, Anhui Province
Grain and Oil Product Quality Supervision and Inspection Station, Yunnan Grain and Oil Scientific Research Institute (Yunnan Grain and Oil Product Quality Supervision and Inspection Center), Shanxi Provincial Inspection
Testing Center (Shanxi Institute of Standards and Metrology), Shaanxi Grain Quality and Safety Center, Qingdao Yuanxin Testing Technology Co., Ltd., Tianjin
Qingdao Branch of Xiang (Tianjin) Quality Technology Service Co., Ltd. and Institute of Food and Nutrition Development of the Ministry of Agriculture and Rural Affairs.
The main drafters of this document are. Cui Hua, Wang Songxue, Xie Gang, Wang Pei, Li Keqiang, Guo Yuting, Wang Jing, Tan Junfeng, Li Sen, Zhang Hongwei, Zhao Liangjuan,
Zhang Yue, Zhang Jianqiao, Shi Jiayuan, Wang Yajun, Zhu Qisi, Hu Bin, Liu Fuying, Wang Xuezheng, Wang Youkai, Wang Lihua, Song Zewei, Zhao Li, Gao Lufei,
Han Juan.
Grain and Oil Inspection. Counting Mold in Grains
Fluorescence rapid detection method
1 Scope
This document specifies the reagents and materials, instruments and equipment, operation steps, result calculation and
Reporting and waste disposal.
This document applies to the enumeration of mold in grains and their products.
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
GB 19489 General requirements for laboratory biosafety
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Enumeration of molds
The sample is processed and cultured under certain conditions to obtain the number of molds in 1g of sample.
3.2
colony-forming unit; CFU
A colony formed by a single bacterial cell or multiple bacterial cells aggregated together growing and multiplying on a solid culture medium.
4 Principle
After the sample dilution passes through the filter membrane, the mold is retained on the surface of the filter membrane. After amplification and cultivation in the culture medium, the non-luminescent enzyme substrate in the fluorescent reagent
The substrate enters the fungus and undergoes enzymatic hydrolysis, releasing the luminescent group into the cytoplasm of the fungus, where the luminescent group accumulates in the cell.
Afterwards, the mold colonies are excited by the excitation light and show fluorescence.
5 Reagents and Materials
Unless otherwise specified, the test water should conform to the specification of Grade 3 water in GB/T 6682.
5.1 Improved mold culture medium. see Appendix A, A.1.
5.2 Physiological saline. see A.2.
5.3 Fluorescent colorimetric solution. see A.3.
5.4 Sterile homogenizing bag or conical flask. capacity 250mL.
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