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Meat and meat products -- Determination of chloramphenicol content
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GB/T 9695.32-2009
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Basic data Standard ID | GB/T 9695.32-2009 (GB/T9695.32-2009) | Description (Translated English) | Meat and meat products -- Determination of chloramphenicol content | Sector / Industry | National Standard (Recommended) | Classification of Chinese Standard | X04 | Classification of International Standard | 67.040 | Word Count Estimation | 10,173 | Date of Issue | 2009-04-08 | Date of Implementation | 2009-05-01 | Regulation (derived from) | National Standard Approval Announcement 2009 No.3 (Total No.143) | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | Summary | This standard specifies the method for the determination of chloramphenicol in poultry meat. This standard applies to the determination of chloramphenicol in poultry meat. This standard detection limit: gas chromatography-mass spectrometry. The detection limit was 0. 2 ��g/kg, enzyme-linked immunosorbent assay for the 0. 05��g/kg. |
GB/T 9695.32-2009: Meat and meat products -- Determination of chloramphenicol content---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Meat and meat products.Determination of chloramphenicol content
ICS 67.040
X04
National Standards of People's Republic of China
Meat and meat products Determination of chloramphenicol
Posted 2009-04-08
2009-05-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
The standard-setting process in reference to the "European Food Analysis CY3.6".
Appendix A of this standard is an informative annex.
This standard by the National Standardization Technical Committee of meat products, eggs and focal points.
This standard was drafted. China Meat Research Center, China Chamber of Commerce Business Standard Centre, Wuhan Disease Control and Prevention
System Center, Jiangyin City Product Quality Supervision, Xiamen Municipal Center for Disease Control.
The main drafters of this standard. Song Yongqing, Zhao Rong, Liang High Road, Guo Wenping Wu Lei, Luo and East, Jin Xiaolei Liu Zhenyu.
Meat and meat products Determination of chloramphenicol
1 Scope
This standard specifies the method for the determination of chloramphenicol in livestock and poultry meat.
This standard applies to the determination of poultry meat chloramphenicol.
The standard detection limit. gas chromatography - mass spectrometry detection limit of 0.2μg/kg; enzyme-linked immunosorbent assay to 0.05μg/kg.
2 Normative references
The following documents contain provisions by GB/T 9695 reference in this part of the provisions of this section. For dated references,
All subsequent amendments (not including errata content) or revisions do not apply to this section, however, encourage the agreement on this section
Whether the parties can study the latest versions of these documents. For undated reference documents, the latest versions apply to this section.
GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)
GB/T 9695.19 Meat and meat products - Sampling Methods
3 Gas chromatography - mass spectrometry (confirmatory method)
3.1 Principle
Chloramphenicol samples extracted with ethyl acetate, n-hexane to remove the fat, the C18 purification, BSTFA + TMCS (99 + 1) derived after
3.2 Reagents and materials
Unless otherwise noted, reagents were analytical grade, the water should be consistent with GB/T 6682 requirements.
3.2.1 chloramphenicol. standard, purity ≥99%.
3.2.2 Methanol. chromatographically pure.
3.2.3 chloroform.
3.2.4 n-hexane. chromatographically pure.
3.2.5 ethyl acetate.
3.2.6 anhydrous sodium sulfate.
3.2.7 sodium chloride.
3.2.8 N, O- bis trimethylsilyl trifluoroacetamide (BSTFA).
3.2.9 trimethylchlorosilane (TMCS).
3.2.10 acetone. chromatography.
3.2.11 toluene.
3.2.12 methanol solution. water + methanol = 8 + 2.
3.2.13 sodium chloride solution (40g/L). Weigh 4.00g of sodium chloride (3.2.7.7), dissolved in water, dilute to 100mL.
3.2.14 methanol - sodium chloride solution. amount of methanol solution (3.2.12) 20mL, sodium chloride solution (3.2.13) 80mL, mix.
3.2.15 mixed derivatizing agent. N, O- bis trimethylsilyl trifluoroacetamide trimethylchlorosilane + + 1 = 99.
3.2.16 chloramphenicol standard stock solution (Ba = 0.1mg/mL). Weigh chloramphenicol standard 0.01g (accurate to 0.0001g), with acetone
Dissolved and set the volume to 100mL. Stock solution stored at 4 ℃ refrigerator, you can use two months.
3.2.17 Chloramphenicol standard solution. According to the test required, appropriate dilution of stock standard solution (2.2.17) with acetone (2.2.10), dubbed
Standard working solution concentration.
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