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GB/T 8314-2013: Tea -- Determination of free amino acids content
Status: Valid GB/T 8314: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 8314-2013 | English | 139 |
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3 days [Need to translate]
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Tea -- Determination of free amino acids content
| Valid |
GB/T 8314-2013
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| GB/T 8314-2002 | English | 199 |
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Tea -- Determination of free amino acids content
| Obsolete |
GB/T 8314-2002
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| GB/T 8314-1987 | English | 199 |
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Tea--Determination of free amino acids content
| Obsolete |
GB/T 8314-1987
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PDF similar to GB/T 8314-2013
Basic data | Standard ID | GB/T 8314-2013 (GB/T8314-2013) | | Description (Translated English) | Tea -- Determination of free amino acids content | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.140.10 | | Word Count Estimation | 6,680 | | Older Standard (superseded by this standard) | GB/T 8314-2002 | | Quoted Standard | GB/T 8302; GB/T 8303; GB/T 8312 | | Regulation (derived from) | National Standards Bulletin 2013 No. 27 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the method for instruments and equipment, reagents and solutions, methods of operation and the results of the determination of free amino acids in tea total calculated. This standard applies to the determination of total free amino | GB/T 8314-2013: Tea -- Determination of free amino acids content---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Tea. Determination of free amino acids content
ICS 67.140.10
X04
National Standards of People's Republic of China
Replacing GB/T 8314-2002
Determination of total free amino acids tea
Issued on. 2013-12-31
2014-06-22 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard replaces GB/T 8314-2002 "Determination of total free amino acids in tea." This standard compared with GB/T 8314-2002, the main
To technical changes are as follows.
--- Refinement added during formulation pH8.0 phosphate buffer;
--- Modify the amino acid standard curve of technical content, so that the sample measurement process is the same;
--- Repeatability. the difference between the two measurement results, the total amount of free amino acids per 100g sample should not exceed 0.1g, modified to repeat
The absolute difference of the same sample obtained under the conditions of the measurement result shall not exceed 10% of the arithmetic mean.
The standard proposed by China Federation of Supply and Marketing Cooperatives.
This standard by the National Standardization Technical Committee of tea (SAC/TC339) centralized.
This standard was drafted. China Federation of Supply and Marketing Cooperatives Hangzhou Tea Research Institute, the National Tea Quality Supervision and Inspection Center.
The main drafters of this standard. Xu Jianfeng, Zhou Weilong, Lu Xiaolei, Wang Jin, Sha Tao.
The previous versions of GB/T 8314 release of case.
--- GB/T 8314-1987, GB/T 8314-2002.
Determination of total free amino acids tea
1 Scope
This standard specifies the apparatus and appliances, reagents and solutions, operating methods and results Determination of free amino acids in tea calculated
method.
This standard applies to the determination of total free amino acid in tea.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB/T 8302 sampling tea
Sample Preparation and Determination of dry matter content GB/T 8303 Tea grated
GB/T 8312 Determination of caffeine in tea
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Free amino acids freeaminoacids
Tea flooding α- amino acids having a free state in the physical form.
Principle 4
α- amino acid at pH8.0 conditions were hot with ninhydrin to form a purple complex measured by spectrophotometry at a specific wavelength
Its content.
5 apparatus and appliances
5.1 Analysis of balance. a sense of the amount of 0.001g.
5.2 Spectrophotometer.
5.3 colorimetric tube. a plug, 25mL.
6 Reagents and solutions
6.1 Unless otherwise indicated, the methods used were of analytical grade reagents (AR), distilled water.
6.2 pH8.0 phosphate buffer
6.2.1 1/15mol/L disodium hydrogen phosphate. Weigh 23.9g twelve - hydrated disodium hydrogen phosphate (Na2HPO4 · 12H2O), add water to dissolve into
1L volumetric flask, dilute to the mark, shake.
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