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GB/T 7628-2008: Determination of vitamin B1 in cereals
Status: Obsolete GB/T 7628: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 7628-2008 | English | 319 |
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Determination of vitamin B1 in cereals
| Obsolete |
GB/T 7628-2008
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| GB/T 7628-1987 | English | 199 |
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Method for the determination of vitamin B1 in cereals
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GB/T 7628-1987
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PDF similar to GB/T 7628-2008
Standard similar to GB/T 7628-2008 GB 7718 GB/T 5532 GB 7718 GB/T 5534 Basic data | Standard ID | GB/T 7628-2008 (GB/T7628-2008) | | Description (Translated English) | Determination of vitamin B1 in cereals | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.060 | | Word Count Estimation | 8,865 | | Date of Issue | 2008-05-27 | | Date of Implementation | 2008-10-01 | | Older Standard (superseded by this standard) | GB/T 7628-1987 | | Quoted Standard | GB 5491; GB/T 6682 | | Regulation (derived from) | National Standard Approval Announcement 2008 No.9 (Total No.122) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the principle of fluorescence spectrophotometer grains of vitamin B1, reagents and materials, equipment, sample preparation, analysis step, calculated results and repeatability. This standard applies to the determination of vitamin B1 in cereals. This standard detection limit of 0. 05 ��g. | GB/T 7628-2008: Determination of vitamin B1 in cereals---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of vitamin B1 in cereals
ICS 67.060
X04
National Standards of People's Republic of China
Replacing GB/T 7628-1987
Determination of Vitamin B1 in cereals
Posted 2008-05-27
2008-10-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard reference AACCMethod86-80 "Thiamine-ThiochromeMethod" (2000, in English).
This standard is GB/T 7628-1987 "Determination of cereal Vitamin B1" amendments.
This standard and GB/T 7628-1987 The main differences are as follows.
--- Test water should comply with the provisions of GB/T 6682 water in three specifications;
--- Provides for sampling, sub-sampling method;
--- Hydrochloric acid hydrolysis to sulfuric acid hydrolysis;
--- Increased use of ion exchange resins;
--- N-butanol solvent to isobutanol solvent;
--- Extract light color, without interfering substances, without purification step;
--- Canceled on a dry basis of the requirements of the measurement results;
--- Increased vitamin B1 content is calculated not by purification of the sample;
--- Increased requirements for the determination of Vitamin B1 repetitive cereal when low levels.
The standard proposed by the National Food Authority.
This standard by the National Standardization Technical Committee OILS.
This standard was drafted. the National Food Authority feed grain Wuhan Quality Supervision and Inspection Center.
The main drafters of this standard. Yang Lin, Liu Xiaomin, Qu Liwen, He Yifan, money Chuang.
This standard replaces the standards previously issued as follows.
--- GB/T 7628-1987.
Determination of Vitamin B1 in cereals
1 Scope
This standard specifies the principle of fluorescence spectrophotometer grains of vitamin B1, reagents and materials, equipment, sample preparation,
Step analysis, the results of calculation and repeatability.
This standard applies to the determination of grains of vitamin B1.
The standard detection limit of 0.05μg.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB 5491 grain and oilseeds testing for sampling, and sample method
GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987)
Principle 3
The sample was dilute sulfuric acid, starch decomposing enzyme solution of vitamin B1 (thiamin, C12H17ON4SCl) is potassium ferricyanide under alkaline conditions
Oxidation of sulfur dye, that is a fluorescent dye. Proportional to the sulfur content of the pigment in the isobutanol in fluorescence intensity in the sample of vitamin B1, and so
Determination of vitamin B1 content.
4 Reagents and materials
Unless otherwise specified, the reagents were of analytical grade, test water should be consistent with GB/T 6682 in the three water specifications.
4.1 hydrochloric acid
Take 205g 340g of anhydrous sodium acetate or hydrated sodium acetate (CH3COONa · 3H2O) dissolved in water and diluted to 1000mL.
4.3 acetic acid solution Ba (CH3COOH) = 3% (volume fraction)
Take 30mL glacial acetic acid diluted with water to 1000mL.
Weigh 250g of potassium chloride dissolved in water and diluted to 1000mL.
Weigh 1g silver nitrate was dissolved in water and dilute to 100mL.
Take 6g amylase preparation (takadiastase), and its activity was equivalent activity or other 1.250 phosphatase, using 2.5mol/L B
Sodium solution (4.2) prepare a suspension, diluted to 100mL, that day prepared the day of use.
4.7 ion exchange agent
4.7.1 active zeolite
The zeolite according to the provisions of the sieve filter layer, weighed through the upper sieve (aperture 0.297mm, 50 mesh), is retained in the lower sieve (aperture
0.177mm, 80 mesh zeolite 100g) in the Buchner funnel, with 3% of hot acetic acid (4.3, about 50 ℃) solution was washed 15min, should make
Acid remains in contact with the zeolite 10min ~ 15min, acetate, vacuum filtration using a vacuum pump; and then 0.25g/mL of hot potassium chloride solution
(4.4, about 50 ℃) 250mL washing; and finally with hot water (about 50 ℃) was washed until the eluate does not appear when using silver nitrate (4.5) inspection
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