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GB/T 46540-2025: Textiles - Determination of microorganisms
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Textiles - Determination of microorganisms
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Standard similar to GB/T 46540-2025 GB/T 45365 | GB/T 45362 | GB/T 45367 | GB/T 46542 | GB/T 46539 | Basic data | Standard ID | GB/T 46540-2025 (GB/T46540-2025) | | Description (Translated English) | Textiles - Determination of microorganisms | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | W04 | | Classification of International Standard | 59.080.01 | | Word Count Estimation | 46,434 | | Date of Issue | 2025-10-31 | | Date of Implementation | 2026-05-01 | | Issuing agency(ies) | State Administration for Market Regulation, Standardization Administration of China | GB/T 46540-2025: Textiles - Determination of microorganisms---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 59.080.01
CCS W04
National Standards of the People's Republic of China
Determination of microorganisms in textiles
Published on 2025-10-31
Implemented on May 1, 2026
State Administration for Market Regulation
The State Administration for Standardization issued a statement.
Table of contents
Preface III
1.Scope 1
2 Normative References 1
3.Terms and Definitions 1
4.Sample Collection and Processing 2
5.Determination of total bacterial count 3
6.Determination of coliform bacteria
7.Determination of Staphylococcus aureus 10
8.Determination of Candida albicans 14
9.Determination of Pseudomonas aeruginosa 18
10.Determination of Group A beta-hemolytic streptococci 23
Appendix A (Normative) Culture Media and Reagents 28
Appendix B (Normative) LAMP Primers 38
Appendix C (Normative) Primers and Probes for Real-Time Fluorescent PCR 39
Appendix D (Informative) Graphs for Determining Positive Results in LAMP and PCR Tests 40
Foreword
This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents".
Drafting.
Please note that some content in this document may involve patents. The issuing organization of this document assumes no responsibility for identifying patents.
This document was proposed by the China National Textile and Apparel Council.
This document is under the jurisdiction of the National Technical Committee on Standardization of Textiles (SAC/TC209).
This document was drafted by. Zhengzhou Customs Technology Center, China Textile Standard (Shenzhen) Testing Co., Ltd., and Nanjing Customs Textile Industry Product Testing Center.
Heart, Zhejiang Yaqinuo Decoration Materials Co., Ltd., Nanchang Customs, China Textile Standard (Zhejiang) Testing Co., Ltd., Jiageng Innovation Laboratory, Ningbo Customs
Technology Center, Jiajia Holding Group Co., Ltd., Hai'an Nantong University Advanced Textile Research Institute, Shenzhen Ellassay Fashion Co., Ltd., Hunan
Huasheng Zhuzhou Cedar Co., Ltd., China Jiliang University, Zhejiang Rongda Fashion Technology Co., Ltd., Guangdong Qiyue Future Technology Co., Ltd., Zhonglian
Quality Inspection (Dongguan) Inspection Technology Co., Ltd., and Ruyudeshui (Hangzhou) Soft Furnishing Customization Co., Ltd.
The main drafters of this document are. Guo Huiqing, Li Ke, Ma Pengfei, Zhang Shuxia, Gui Jiaxiang, Xie Tangtang, Zheng Yue, Fu Kejie, Ding Youchao, and Yu Jianying.
Zhou Yuhang, Liu Xin, Yuan Qiyu, Zhang Wei, Lou Danli, Huang Zhouyong, Zhang Hewei, Zhou Huyun, Yan Ying, Huang Fukai, Sheng Fangming, Zhu Guoquan, Chen Haiyang.
Determination of microorganisms in textiles
Warning. Laboratory biosafety management and facility conditions for conducting microbial testing experiments must meet the requirements of GB 19489.
Personnel using this document should have practical experience working in a microbiology laboratory and are responsible for taking appropriate safety and health protection measures to ensure that...
It complies with relevant national laws and regulations.
1 Scope
This document describes the sample collection and pretreatment for microbial assays in textiles, as well as the determination of total bacterial count, coliforms, and aureus in textiles.
Methods for the determination of Staphylococcus, Candida albicans, Pseudomonas aeruginosa, and beta-hemolytic streptococci.
This document applies to all types of textiles.
2 Normative references
The contents of the following documents, through normative references within the text, constitute essential provisions of this document. Dated citations are not included.
For references to documents, only the version corresponding to that date applies to this document; for undated references, the latest version (including all amendments) applies.
This document.
GB/T 8170 Rules for rounding off numerical values and the representation and determination of limiting values
GB 19489 General Requirements for Laboratory Biosafety
GB/T 36433-2018 Textiles - Quantitative Analysis of DNA from Mixtures of Cashmere and Sheep Wool - Fluorescent PCR Method
3 Terms and Definitions
The terms and definitions defined in GB/T 36433-2018, as well as the following terms and definitions, apply to this document.
3.1
Colony-forming units (CFU)
The unit for determining the concentration of viable bacteria in a sample is based on the number of colonies formed on a solid culture medium.
3.2
Under an isothermal environment of 60℃~65℃, DNA is in a dynamic equilibrium state. At this temperature, four specific primers are used with a high-performance...
Active chain replacement DNA polymerase is a technology that enables DNA to undergo nucleic acid amplification in a short time.
Note. The reaction produces a large amount of amplification products, namely magnesium pyrophosphate, which is a white precipitate. The presence or absence of the white precipitate can be determined by visually observing it.
3.3
Real-time fluorescence quantitative PCR; PCR
A fluorescent group is added to the PCR reaction system, and the accumulation of fluorescence signal is used to monitor the entire PCR process in real time. Then, the fluorescence signal is used to analyze the results of the unprocessed PCR.
DNA amplification methods that use known templates for quantitative or qualitative analysis.
[Source. GB/T 36433-2018, 3.2]
3.4
Ct value cyclethreshold
The number of cycles required for the fluorescence signal in each reaction tube to reach the set threshold.
[Source. GB/T 36433-2018, 3.5]
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