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GB/T 45548-2025 English PDF

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GB/T 45548-2025: Determination of isotope abundance of deuterated reagents - Nuclear magnetic resonance spectroscopy
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Basic data

Standard ID GB/T 45548-2025 (GB/T45548-2025)
Description (Translated English) Determination of isotope abundance of deuterated reagents - Nuclear magnetic resonance spectroscopy
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard A40
Classification of International Standard 07.080
Word Count Estimation 22,241
Date of Issue 2025-04-25
Date of Implementation 2025-04-25
Issuing agency(ies) State Administration for Market Regulation, China National Standardization Administration

GB/T 45548-2025: Determination of isotope abundance of deuterated reagents - Nuclear magnetic resonance spectroscopy


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ICS 07.080 CCSA40 National Standard of the People's Republic of China Determination of isotopic abundance of deuterated reagents Nuclear magnetic resonance spectroscopy Released on 2025-04-25 2025-04-25 implementation State Administration for Market Regulation The National Standardization Administration issued

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents" Drafting. Please note that some of the contents of this document may involve patents. The publishing organization of this document does not assume the responsibility for identifying patents. This document was proposed and coordinated by the National Technical Committee for Biochemical Testing Standardization (SAC/TC387). This document was drafted by. Shanghai Research Institute of Chemical Industry Co., Ltd., China Testing Technology Research Institute, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences Institute of Metrology and Testing Technology, Shanghai Institute of Metrology and Testing Technology, Ningbo Cuiying Chemical Technology Co., Ltd., East China University of Science and Technology, China Institute of Metrology, Wuhan University of Science and Technology Hanzhong Science Oxford Spectroscopy Technology Co., Ltd., East China Normal University, Shenzhen Shenjian Group Medical Testing Laboratory, Guangzhou Jiatu Technology Co., Ltd. Company, Shanghai Kangernuo Biopharmaceutical Co., Ltd. The main drafters of this document are. Lei Wen, Xie Long, Sun Wen, Zhou Lihua, Wu Tao, Yu Jinglan, Wang Wei, Guo Yinlong, Zhao Yameng, Gao Zhanghua, Li Jie, Wu Liqing, Ma Lixia, Xu Zengyi, Zhang Weibing, Yan Xianzhong, Zhang Lei, Liu Yahui, Shan Yifan, Liang Dacheng, Wu Jian, Gan Li, Shen An, Xiao Weimin, Yang Fan, Wu Gaosheng. Determination of isotopic abundance of deuterated reagents Nuclear magnetic resonance spectroscopy

1 Scope

This document describes a method for determining the isotopic abundance of deuterated reagents using nuclear magnetic resonance spectroscopy. This document applies to chloroform-D, dichloromethane-D2, chlorobenzene-D5, iodobenzene-D5, bromobenzene-D5, acetone-D6, benzene-D6, toluene-D8, methanol- D4, ethanol-D6, heavy water, dimethyl sulfoxide-D6, tetrahydrofuran-D8, p-toluenesulfonyl methyl ester-D3, bromobutrol-D9, di(2-phthalic acid) Butoxyethyl) ester-D4, methyl parahydroxybenzoate-branched-D3, isovanillin-D3, dimethyl phthalate-D4, ethanol-D, acetic acid-D Isotope abundance determination of 21 deuterated reagents (purity ≥ 98%). For the determination of isotope abundance of other deuterated reagents, please refer to this document.

2 Normative references

This document has no normative references.

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Stable isotope An isotope of an element that does not undergo or is very unlikely to undergo radioactive decay, that is, an isotope of an element with a half-life greater than 1015 years. [Source. GB/T 37750-2019, 3.1.12, modified] 3.2 Isotope abundance The percentage of atoms of a particular isotope in a mixture of isotopes of an element to the total number of atoms of that element. Note. It is expressed as atom% A, where A is the code for the element symbol of the specific isotope. [Source. GB/T 37750-2019, 3.1.13, modified] 3.3 Deuterated reagent A reagent that allows identification of a compound in which one or more hydrogen atoms have been replaced by a stable isotope, deuterium (2H or D). [Source. GB/T 37750-2019, 3.3.11] 3.4 Deuterated sites A site in a compound formed when hydrogen atoms with different chemical shifts are replaced by deuterium atoms. Note. For example, benzene-D6 has a single deuterium-labeled site; toluene-D8 has multiple deuterium-labeled sites, including two deuterium-labeled sites. a deuterium-labeled methyl group and a deuterium-labeled phenyl group. 3.5 Deuterium-labeled compound A compound labeled with deuterium atoms replacing all hydrogen atoms in the compound molecule.

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