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GB/T 45208-2025: Determination of paprika red in feeds - High performance liquid chromatography
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 45208-2025 | English | 199 |
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Determination of paprika red in feeds - High performance liquid chromatography
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GB/T 45208-2025
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Basic data | Standard ID | GB/T 45208-2025 (GB/T45208-2025) | | Description (Translated English) | Determination of paprika red in feeds - High performance liquid chromatography | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B46 | | Classification of International Standard | 65.120 | | Word Count Estimation | 10,152 | | Date of Issue | 2025-01-24 | | Date of Implementation | 2025-08-01 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 45208-2025: Determination of paprika red in feeds - High performance liquid chromatography---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 65.120
CCSB46
National Standard of the People's Republic of China
Determination of Capsanthin in Feed by HPLC
Released on 2025-01-24
2025-08-01 Implementation
State Administration for Market Regulation
The National Standardization Administration issued
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting.
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document was proposed and coordinated by the National Feed Industry Standardization Technical Committee (SAC/TC76).
This document was drafted by. Sichuan Well Testing Technology Co., Ltd., Shandong Animal Products Quality and Safety Center, Guangzhou Zhitech Biotechnology
Technology Co., Ltd., Shandong Feed and Veterinary Drug Quality Inspection Center, and Chenguang Biotechnology Group Co., Ltd.
The main drafters of this document are. Zhang Yun, Zhang Fengping, Sun Yanjun, Gong Lingling, Tang Huaying, Zhang Wei, Wang Yingying, Li Junling, Chen Bing, Tang Jiao, Zhang Kun,
Li Huirong, Song Weiguang, and Wu Jinyu.
Determination of Capsanthin in Feed by HPLC
Warning---People who use this document should have practical experience in formal laboratory work. This document does not address all possible safety issues.
The user is responsible for taking appropriate safety and health measures and ensuring that the conditions are met in accordance with relevant national regulations.
1 Scope
This document describes a method for the determination of capsanthin in feeds by HPLC.
This document applies to capsanthin (the sum of capsanthin and capsorubin) in compound feed, concentrated feed and vitamin premix feed.
Measurement of the
In this document, the detection limits of capsanthin and capsanthin are both 0.05 mg/kg, and the quantification limits are both 0.1 mg/kg.
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
GB/T 20195 Preparation of animal feed samples
3 Terms and definitions
There are no terms or definitions that require definition in this document.
4 Principle
Capsanthin and capsanthorubin in the sample were extracted with acetonitrile and determined by high performance liquid chromatography and quantified by external standard method.
5 Reagents or materials
Unless otherwise specified, only analytical grade reagents were used.
5.1 Water. GB/T 6682, Grade 1.
5.2 Acetonitrile. chromatographic grade.
5.3 Phosphoric acid solution (0.3%, volume fraction). Pipette 1.5 mL of phosphoric acid, dilute to 500 mL with water, and mix well.
5.4 Capsanthin and capsanthin standard stock solution (100 μg/mL). weigh capsanthin (CAS No.. 465-42-9, purity ≥
95%) and capsanthin (CAS No.. 470-38-2, purity ≥ 95%) standard products 10 mg each (accurate to 0.01 mg) were placed in
Place in a 100 mL brown volumetric flask, dissolve with acetonitrile (5.2), make up to volume, and mix well. Store at 2°C~8°C, valid for 1 month.
5.5 Mixed standard intermediate solution (10 μg/mL). Accurately pipette 1 mL of capsanthin and capsanthin standard stock solutions (5.4) respectively.
In a 10 mL brown volumetric flask, dilute with acetonitrile (5.2), make up to volume, and mix well. Prepare before use.
5.6 Mixed standard series solution. Accurately transfer an appropriate amount of mixed standard intermediate solution (5.5) into a 10 mL brown volumetric flask and add acetonitrile (5.2)
Dilute, fix volume, mix, and prepare mass concentrations of 0.02μg/mL, 0.1μg/mL, 0.5μg/mL, 1μg/mL, 2μg/mL and
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