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GB/T 44742-2024: Determination of sea cucumber polysaccharides in sea cucumber and its products - High performance liquid chromatography
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 44742-2024 | English | 209 |
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Determination of sea cucumber polysaccharides in sea cucumber and its products - High performance liquid chromatography
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GB/T 44742-2024
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Basic data
| Standard ID | GB/T 44742-2024 (GB/T44742-2024) |
| Description (Translated English) | Determination of sea cucumber polysaccharides in sea cucumber and its products - High performance liquid chromatography |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B51 |
| Classification of International Standard | 67.120 |
| Word Count Estimation | 10,151 |
| Date of Issue | 2024-10-26 |
| Date of Implementation | 2025-05-01 |
| Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 44742-2024: Determination of sea cucumber polysaccharides in sea cucumber and its products - High performance liquid chromatography
---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT44742-2024
ICS 67.120
CCSB51
National Standard of the People's Republic of China
Determination of sea cucumber polysaccharides in sea cucumber and its products
High-performance liquid chromatography
Released on October 26, 2024
Implementation on May 1, 2025
State Administration for Market Regulation
The National Standardization Administration issued
Preface
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting.
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document is proposed and coordinated by the China National Institute of Standardization.
This document was drafted by. Ocean University of China, China National Institute of Standardization, Shandong Haodangjia Ocean Development Co., Ltd., Dalian Xiaoqin
Food Co., Ltd., Qingdao Marine Food Nutrition and Health Innovation Research Institute, Quanzhou Marine Biological Industry Research Institute, SGS-CSTC Standards Technical Services Co., Ltd.
Co., Ltd. Dalian Branch, Zhongshan Hongli Healthy Food Industry Research Institute Co., Ltd.
The main drafters of this document are. Xue Changhu, Fan Yan, Wang Yuming, Mao Xiangchao, Hou Hu, Xi Xingjun, Hu Wei, Liang Weigang, Sun Yongjun, Li Chao, He Chunli,
Feng Tingyu, Li Ruoshu, Liu Shoufeng, Luan Wenwen, and Lei Minzhi.
Determination of sea cucumber polysaccharides in sea cucumber and its products
High-performance liquid chromatography
1 Scope
This document describes a method for the determination of sea cucumber polysaccharides in sea cucumbers and their products using high performance liquid chromatography.
This document is applicable to the determination of sea cucumber polysaccharides in sea cucumbers and sea cucumber powder.
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
A type of polysaccharide composed of sea cucumber fucoidan sulfate and sea cucumber chondroitin sulfate.
Note. Sea cucumber fucoidan sulfate is a linear sulfated polysaccharide formed by the polymerization of L-fucose; sea cucumber chondroitin sulfate is a type of polysaccharide with fucose side chains.
The substituted sulfated polysaccharide is mainly composed of D-acetylgalactosamine, D-glucuronic acid and L-fucose.
4 Principles
The sample was enzymatically hydrolyzed, potassium acetate precipitated, and acid hydrolyzed to obtain sea cucumber polysaccharide hydrolyzate. The fucose in the hydrolyzate was mixed with 1-phenyl-3-methyl-5-pyridinium chloride.
The product was separated by C18 column and determined by high performance liquid chromatography equipped with UV detector.
The content of sea cucumber polysaccharides in the sample was identified by the fucose content.
5 Reagents and Materials
5.1 Reagents
5.1.1 Water. GB/T 6682, Grade 1.
5.1.2 Papain. Food grade, enzyme activity ≥ 100,000 U/g.
5.1.3 Ethylenediaminetetraacetic acid (C10H16N2O8).
5.1.4 Cysteine hydrochloride (H-Cys-OHHCl).
5.1.5 Potassium acetate (CH3COOK).
5.1.6 Chloroform (CHCl3).
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